Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins
文献类型：期刊论文
| 作者 | Lu, Congcong1,2; Zhang, Kai1,2,3,4; Zhang, Yi5,6; Tan, Minjia5,6 ; Li, Yijun1,2; He, Xiwen1,2; Zhang, Yukui1,2,7
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2014-12-12 |
| 卷号 | 1372页码:34-41 |
| ISSN号 | 0021-9673 |
| 关键词 | Proteomics HPLC-MS/MS Chemical probe Target proteins Vorinostat |
| DOI | 10.1016/j.chroma.2014.10.098 |
| 文献子类 | Article |
| 英文摘要 | Inhibitors of histone deacetylases (HDACs) have been considered to be new anticancer agents. As a key inhibitor of HDAC, vorinostat can cause growth arrest and death of a broad of transformed cells and interact with a variety of substrates. A comprehensive analysis of proteins interacting with HDAC inhibitors is of great importance in understanding molecular mechanisms of the drugs. Here, we reported the preparation and characterization of vorinostat-coated beads for profiling of novel target proteins of vorinostat (a key HDAC inhibitor). The enriched proteins were further analyzed by HPLC-MS/MS. Besides the known substrates, there were also several novel enriched protein candidates, one of which was a metalloenzyme a-enolase (ENO-1). According to our best knowledge, it is the first time that ENO-1 has been detected as a potential target of vorinostat through chemoproteomics approach. Further competition analysis indicated that ENO-1 may be co-enriched as a substrate complex. Our results demonstrated that the chemical probe combined with proteomics approach may be developed as a potential tool to identify target proteins of drugs. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | HISTONE DEACETYLASE INHIBITORS ; LYSINE ACETYLATION ; MOLECULAR-MECHANISMS ; HYDROXAMIC ACID ; CANCER CELLS ; HDAC ; SAHA ; THERAPIES ; COMPLEXES |
| 资助项目 | National Basic Research Program of China[2012CB910601] ; National Basic Research Program of China[2013CB910903] ; National Natural Science Foundation of China[21275077] ; National Natural Science Foundation of China[90919008] ; Tianjin Municipal Science and Technology Commission[14JCYBJC24000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000347020900004 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276804]  |
| 专题 | 化学蛋白质组学研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhang, Kai |
| 作者单位 | 1.Nankai Univ, State Key Lab Med Chem Biol, Tianjin 300071, Peoples R China; 2.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China; 3.Tianjin Med Univ, Basic Med Sch, Dept Biochem & Mol Biol, Tianjin 300070, Peoples R China; 4.Tianjin Med Univ, Basic Med Sch, Tianjin Key Lab Med Epigenet, Tianjin 300070, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Chem Prote Ctr, Shanghai 201203, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 7.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Congcong,Zhang, Kai,Zhang, Yi,et al. Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins[J]. JOURNAL OF CHROMATOGRAPHY A,2014,1372:34-41. |
| APA | Lu, Congcong.,Zhang, Kai.,Zhang, Yi.,Tan, Minjia.,Li, Yijun.,...&Zhang, Yukui.(2014).Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins.JOURNAL OF CHROMATOGRAPHY A,1372,34-41. |
| MLA | Lu, Congcong,et al."Preparation and characterization of vorinostat-coated beads for profiling of novel target proteins".JOURNAL OF CHROMATOGRAPHY A 1372(2014):34-41. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。