Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition
文献类型：期刊论文
| 作者 | Zhao, Jiaohong1,2; Chen, Liping2,3,4; Gao, Fudan2; Wang, Quanxi2; Qiu, Zijian1; Ma, Weimin2 |
| 刊名 | ACTA BIOCHIMICA ET BIOPHYSICA SINICA
 |
| 出版日期 | 2014-10 |
| 卷号 | 46期号:10页码:911-916 |
| 关键词 | state transition phycobilisome Synechocystis 6803 |
| ISSN号 | 1672-9145 |
| DOI | 10.1093/abbs/gmu072 |
| 文献子类 | Article |
| 英文摘要 | State transition is a short-term balance mechanism of energy distribution between photosystem II (PSII) and PSI. Although light-induced state transition in cyanobacteria has been suggested to depend completely on the phycobilisome (PBS) movement between PSII and PSI, the biochemical evidence has not been clearly shown. In this study, we locked the association of PBS with PSII or PSI using glycinebetaine when cells attain State 1 or 2 by exposure to light of blue or green, respectively. Subsequently, the PBS-reaction centers were resolved by blue native polyacrylamide gel electrophoresis and two-dimensional electrophoresis, and then identified by western blot analysis. The results showed that in wild-type (WT) Synechocystis sp. strain PCC 6803, the PBS core always co-migrates with the PSII dimer during light-induced State 1-State 2 transition, but its rod leaves the PSII dimer in State 2 regardless of its co-migration in State 1. In the light-induced State 2, the co-migration of PBS rod with PSI trimer was observed in WT, but not in Delta ndhB (M55), a State-2-transition-deficient mutant. This study first provided the biochemical evidence for the association of PBS with photosystems during cyanobacterial state transition. |
| WOS关键词 | EXCITATION-ENERGY TRANSFER ; STRAIN PCC 6803 ; SPIRULINA-PLATENSIS ; PORPHYRIDIUM-CRUENTUM ; THYLAKOID MEMBRANES ; ELECTRON-TRANSPORT ; NDH-1 COMPLEXES ; CHLOROPHYLL ; PROTEIN ; FLUORESCENCE |
| 资助项目 | National Natural Science Foundation of China[31370270] ; National Basic Research Program of China[2009CB118500] ; Shanghai Education Committee[12ZZ132] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000343053500010 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276882]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Qiu, Zijian |
| 作者单位 | 1.Harbin Normal Univ, Coll Life Sci & Technol, Harbin 150025, Peoples R China; 2.Shanghai Normal Univ, Coll Life & Environm Sci, Shanghai 200234, Peoples R China; 3.Chinese Acad Sci, Key Lab Receptor Res, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Jiaohong,Chen, Liping,Gao, Fudan,et al. Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2014,46(10):911-916. |
| APA | Zhao, Jiaohong,Chen, Liping,Gao, Fudan,Wang, Quanxi,Qiu, Zijian,&Ma, Weimin.(2014).Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,46(10),911-916. |
| MLA | Zhao, Jiaohong,et al."Identification of biochemical association of phycobilisome with photosystems in cyanobacterial state transition".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 46.10(2014):911-916. |
入库方式： OAI收割
来源：上海药物研究所
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