Enantioselective HPLC determination and pharmacokinetic study of secnidazole enantiomers in rats
文献类型：期刊论文
| 作者 | Du, Jiangbo; Zhang, Yifan ; Chen, Yao; Liu, Dongqin; Chen, Xiaoyan; Zhong, Dafang
|
| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
 |
| 出版日期 | 2014-08-15 |
| 卷号 | 965页码:224-230 |
| 关键词 | Secnidazole Enantiomers Enantioselective high-performance liquid chromatography Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2014.06.036 |
| 文献子类 | Article |
| 英文摘要 | Secnidazole is a long-lasting nitroimidazole antimicrobial agent that is used as racemic mixture in clinical settings. We developed and validated an enantioselective high-performance liquid chromatography method to determine secnidazole enantiomers in rat plasma. Secnidazole enantiomers and S-(-)-ornidazole (internal standard) were extracted from 50 mu L of rat plasma using diethyl ether-dichloromethane (3:2, v/v). Baseline resolution (R-s = 2.45) was achieved within 7.0 min on a Chiral-AGP column (150 mm x 4.0 mm, 5 mu m) at 20 degrees C. The mobile phase consisted of 10 mM ammonium acetate-methanol (96:4, v/v) and was delivered at a flow rate of 0.5 mL/min with ultraviolet detection at 318 nm. The method was linear over the concentration range 0.500-100 mu g/mL for both enantiomers. The lower limit of quantification was 0.500 mu g/mL for both enantiomers. The relative standard deviation values for intra- and inter-day precision were 0.8-8.6 and 1.8-8.2% for S-(+)-secnidazole and R-(-)-secnidazole, respectively. The relative error values of accuracy ranged from -7.8 to 1.1% for S-(+)-secnidazole and from -7.3 to -0.1% for R-(-)-secnidazole. The method was successfully used to determine the pharmacokinetic properties of secnidazole enantiomers in rats after administration of the racemate and individual enantiomers. The pharmacokinetic results indicate that the disposition of secnidazole enantiomers is not stereoselective and chiral inversion and enantiomer-enantiomer interaction do not occur in rats. (C) 2014 Elsevier B.V. All rights reserved. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; CHIRAL STATIONARY PHASES ; CENTRAL-NERVOUS-SYSTEM ; ORNIDAZOLE ENANTIOMERS ; LIQUID-CHROMATOGRAPHY ; HUMAN PLASMA ; CLINICAL-PHARMACOLOGY ; ENANTIOSEPARATIONS ; MORINIDAZOLE ; MICE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000340313600031 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/276945]  |
| 专题 | 上海药物代谢研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhong, Dafang |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du, Jiangbo,Zhang, Yifan,Chen, Yao,et al. Enantioselective HPLC determination and pharmacokinetic study of secnidazole enantiomers in rats[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2014,965:224-230. |
| APA | Du, Jiangbo,Zhang, Yifan,Chen, Yao,Liu, Dongqin,Chen, Xiaoyan,&Zhong, Dafang.(2014).Enantioselective HPLC determination and pharmacokinetic study of secnidazole enantiomers in rats.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,965,224-230. |
| MLA | Du, Jiangbo,et al."Enantioselective HPLC determination and pharmacokinetic study of secnidazole enantiomers in rats".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 965(2014):224-230. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。