中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 化学蛋白质组学研究中心
Comprehensive profiling of lysine acetylome in Staphylococcus aureus

文献类型：期刊论文

作者Zhang Yi1,2; Wu ZhiXiang1,2; Wan XueLian1,2; Liu Ping1,2; Zhang JiBao2,3; Ye Yang2,3; Zhao YingMing1,2,4; Tan MinJia1,2
刊名SCIENCE CHINA-CHEMISTRY
出版日期2014-05
卷号57期号:5页码:732-738
关键词proteomics post-translational modification acetylation Staphylococcus aureus
ISSN号1674-7291
DOI10.1007/s11426-014-5100-4
文献子类Article
英文摘要The Gram-positive bacterium Staphylococcus aureus (S. aureus) is a wide spread common opportunistic pathogen that causes a wide variety of infectious diseases, from benign skin infections to life-threatening diseases such as the methicillin-resistant Staphylococcus aureus (MRSA) infection. Although emerging evidence suggests that lysine acetylation may play critical roles in bacterial physiology, the atlas of acetylome in S. aureus has not been studied. To comprehensively profile protein lysine acetylation in S. aureus, we used an integrated approach that combined immune affinity peptide enrichment using anti-lysine acetylation antibody, high-pH HPLC fractionation, and HPLC/mass spectrometry analysis. This study led to the identification of 1361 non-redundant acetylation sites on 412 proteins found in a search of S. aureus protein database extracted from the Swiss-Prot database. We further performed bioinformatic analysis to characterize this modification, including gene ontology annotation, protein-protein interaction, and domain analysis of the acetylation sites. We found that the acetylated proteins were enriched in multiple biological pathways, such as ribosomal function and energy metabolism. Our data provides a rich source for functional studies of lysine acetylation in S. aureus.
WOS关键词LARGE GENE LISTS ; CHROMATIN MODIFICATIONS ; METABOLIC ENZYMES ; ESCHERICHIA-COLI ; ACETYLATION ; IDENTIFICATION ; PROTEINS ; DATABASE ; REVEALS
资助项目National Science and Technology Major Project of the Ministry of Science and Technology of China[2012ZX09301001-007] ; National Natural Science Foundation of China[31370814] ; Shanghai Pujiang Program[13PJ1410300] ; China Postdoctoral Science Foundation[2013M531236] ; China Postdoctoral Science Foundation[2013M541567]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000335735200011
出版者SCIENCE PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/277097]  
专题化学蛋白质组学研究中心
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Tan MinJia
作者单位1.Chinese Acad Sci, Chem Prote Ctr, Shanghai 201203, Peoples R China;
2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China;
3.Chinese Acad Sci, Nat Prod Chem Dept, Shanghai 201203, Peoples R China;
4.Univ Chicago, Ben May Dept Canc Res, Chicago, IL 60637 USA
推荐引用方式
GB/T 7714		 Zhang Yi,Wu ZhiXiang,Wan XueLian,et al. Comprehensive profiling of lysine acetylome in Staphylococcus aureus[J]. SCIENCE CHINA-CHEMISTRY,2014,57(5):732-738.
APA Zhang Yi.,Wu ZhiXiang.,Wan XueLian.,Liu Ping.,Zhang JiBao.,...&Tan MinJia.(2014).Comprehensive profiling of lysine acetylome in Staphylococcus aureus.SCIENCE CHINA-CHEMISTRY,57(5),732-738.
MLA Zhang Yi,et al."Comprehensive profiling of lysine acetylome in Staphylococcus aureus".SCIENCE CHINA-CHEMISTRY 57.5(2014):732-738.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。