Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta 1-induced activation of kidney fibroblasts
文献类型：期刊论文
| 作者 | Zhu, Rong1; Zheng, Rong1; Deng, Yueyi1; Chen, Yiping1; Zhang, Shuwei2 |
| 刊名 | PHYTOMEDICINE
 |
| 出版日期 | 2014-02-15 |
| 卷号 | 21期号:3页码:372-378 |
| 关键词 | Ergosterol peroxide Cordyceps cicadae TGF-beta 1 Chronic kidney disease Fibroblast-to-myofibroblast transdifferentiaton |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2013.08.022 |
| 文献子类 | Article |
| 英文摘要 | Chronic kidney disease is a growing public health problem with an urgent need for new pharmacological agents. Ergosterol peroxide (EP) is the major sterol produced by Cordyceps cicadae Shing (C cicadae), a widely used traditional Chinese medicine. C cicadae has been used to treat many kinds of diseases and has a potential benefit on renoprotection. This study aimed to investigate the anti-fibrotic effects of EP as well as the underlying mechanisms. A normal rat kidney fibroblast cell line (NRK-49F) was stimulated to undergo fibroblast activation by transforming growth factor-beta 1 (TGF-beta 1) and EP treatment was applied to explore its potential anti-fibrotic effects. Cell proliferation was investigated using MTT analysis. Fibrosis-associated protein expression was analyzed using immunohistochemistry and/or Western blotting. EP treatment attenuated TGF-beta 1-induced renal fibroblast proliferation, expression of cytoskeleton protein and CTGF, as well as ECM production. Additionally, EP blocked TGF-beta 1-stimulated phosphorylation of ERK1/2, p38 and JNK pathway. Moreover, the TGF-beta 1-induced expression of fibronectin was attenuated by either inhibition of MAPKs or by EP treatment. In conclusion, our findings demonstrate that EP is able to suppress TGF-beta 1-induced fibroblasts activation in NRK-49F. This new information provides a line of theoretical evidence supporting the use of C cicadae in the intervention of kidney disease and suggests that EP has the potential to be developed as a therapeutic agent to prevent renal fibrosis. (C) 2013 Elsevier GmbH. All rights reserved. |
| WOS关键词 | COLON ADENOCARCINOMA CELLS ; HUMAN MESANGIAL CELLS ; TISSUE GROWTH-FACTOR ; RENAL FIBROBLASTS ; MAP KINASE ; FIBROSIS ; DISEASE ; MYOFIBROBLASTS ; EXPRESSION ; RESPONSES |
| 资助项目 | Innovation Team of Shanghai Municipal Education Commission[00000000] ; Shanghai University of Traditional Chinese Medicine[00000000] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000334012400025 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277183]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Deng, Yueyi |
| 作者单位 | 1.Shanghai Univ Tradit Chinese Med, Longhua Hosp, Dept Nephrol, Shanghai 200032, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Rong,Zheng, Rong,Deng, Yueyi,et al. Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta 1-induced activation of kidney fibroblasts[J]. PHYTOMEDICINE,2014,21(3):372-378. |
| APA | Zhu, Rong,Zheng, Rong,Deng, Yueyi,Chen, Yiping,&Zhang, Shuwei.(2014).Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta 1-induced activation of kidney fibroblasts.PHYTOMEDICINE,21(3),372-378. |
| MLA | Zhu, Rong,et al."Ergosterol peroxide from Cordyceps cicadae ameliorates TGF-beta 1-induced activation of kidney fibroblasts".PHYTOMEDICINE 21.3(2014):372-378. |
入库方式： OAI收割
来源：上海药物研究所
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