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Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling

文献类型：期刊论文

作者Wu JunZhou2,3; Wang Jie2,3; Tang XinJing1,2,3,4
刊名SCIENCE CHINA-CHEMISTRY
出版日期2014-02
卷号57期号:2页码:322-328
关键词photolabile nucleotides enzymatic incorporation nucleotide analogues fluorescent labeling
ISSN号1674-7291
DOI10.1007/s11426-013-5034-2
文献子类Article
英文摘要Two new photolabile nucleotide analogues with furan-fused deoxyuridine were synthesized through Sonogashira coupling. Their enzymatic incorporation into DNA was evaluated with two DNA polymerases (Taq and Deep vent exo-) by polymerase chain reaction (PCR). Deep vent exo- recognized both nucleotides as substrates for primer extension, while Taq was much less proficient. Light irradiation of PCR products released the amino and carboxyl moieties of DNA. Further labeling with fluorescein isothiocyanate for a long DNA construct with F-dUnTP incorporation was successfully achieved.
WOS关键词NUCLEOSIDE TRIPHOSPHATES ; NUCLEOTIDES ; OLIGONUCLEOTIDES
资助项目National Natural Science Foundation of China[21072015] ; National Basic Research Program of China (973 Program)[2012CB720600] ; Program for New Century Excellent Talents in University[NCET-10-0203] ; State Key Laboratory of Drug Research[SIMM1106KF-15]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000330681700015
出版者SCIENCE PRESS
源URL[http://119.78.100.183/handle/2S10ELR8/277209]  
专题新药研究国家重点实验室
中科院受体结构与功能重点实验室
通讯作者Tang XinJing
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China;
3.Peking Univ, Sch Pharmaceut Sci, Beijing 100191, Peoples R China;
4.Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
推荐引用方式
GB/T 7714		 Wu JunZhou,Wang Jie,Tang XinJing. Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling[J]. SCIENCE CHINA-CHEMISTRY,2014,57(2):322-328.
APA Wu JunZhou,Wang Jie,&Tang XinJing.(2014).Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling.SCIENCE CHINA-CHEMISTRY,57(2),322-328.
MLA Wu JunZhou,et al."Synthesis of photolabile dUTP analogues and their enzymatic incorporation for DNA labeling".SCIENCE CHINA-CHEMISTRY 57.2(2014):322-328.

入库方式： OAI收割

来源：上海药物研究所

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