Fructopyrano-(1 -> 4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner
文献类型：期刊论文
| 作者 | Liu, Minghua2; Yang, Sijin3; Zhang, Dan2; Shui, Pixian2; Song, Shanshan4; Yao, Jian1; Dai, Yong5; Sun, Qin2 |
| 刊名 | INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
 |
| 出版日期 | 2014 |
| 卷号 | 7期号:11页码:3859-3869 |
| 关键词 | Vascular endothelial growth factor vascular endothelial growth factor receptor liver cancer fructopyrano-(1 -> 4)-glucopyranose |
| ISSN号 | 1940-5901 |
| 文献子类 | Article |
| 英文摘要 | Purpose: To investigate whether fructopyrano-(1 -> 4)-glucopyranose (FG) inhibits the proliferation of liver cancer cells and angiogenesis in a vascular endothelial growth factor (VEGF)/vascular endothelial growth factor receptor (VEGFR) dependent manner. Methods: Bel-7402, HepG2 and SMMC-7721 cells with high expression of VEGF and VEGFR were screened. Bel-7402 cells and human microvascular endothelial cells (HMEC) were treated with FG for 48 h. CCK-8 assay was used to detect cell proliferation. Wound healing assay was used to investigate effect of FG on the migration of HMECs. Tube formation assay was done to test influence of FG on the angiogenesis of HMECs, and qRT-PCR and western blot assay were performed to detect mRNA and protein expression of VEGF, Fit-1 and KDR. Nude mice were inoculated with Bel-7402 cells, and influence of FG on tumor growth, microvessel density (MVD) and VEGF expression in tumor was investigated. Results: Bel-7402 cells had a significantly higher expression of VEGF and VEGFR when compared with HepG2 cells and SMMC-7721 cells. FG could markedly reduce the mRNA and protein expressions of VEGF, Fit-1 and KDR in Bel-7402 cells and inhibit the proliferation of Bel-7402 cells in a concentration dependent manner. In addition, FG was able to remarkably inhibit the proliferation, migration and angiogenesis of HMECs, exerting anti-angiogenetic effect. In cancer-bearing nude mice, FG was found to inhibit the tumor growth, reduce MVD in tumors and decrease the VEGF in tumors. Conclusions: FG can inhibit proliferation of liver cancer cells and suppression angiogenesis in liver cancer in a VEGF/VEGFR dependent manner. |
| WOS关键词 | ENDOTHELIAL GROWTH-FACTOR ; HEPATOCELLULAR-CARCINOMA |
| 资助项目 | National Natural Science Foundation of China[30873381] |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000348302100005 |
| 出版者 | E-CENTURY PUBLISHING CORP |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277249]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Sun, Qin |
| 作者单位 | 1.Peoples Hosp Luzhou City, Dept Hepatobiliary Surg, Luzhou 646000, Peoples R China; 2.Luzhou Med Coll, Dept Pharm, Luzhou 646000, Peoples R China; 3.Luzhou Med Coll, Affiliated Hosp Tradit Chinese Med, Luzhou 646000, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China; 5.Sichuan Police Coll, Dept Criminal Sci & Technol, Luzhou 646000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Minghua,Yang, Sijin,Zhang, Dan,et al. Fructopyrano-(1 -> 4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,2014,7(11):3859-3869. |
| APA | Liu, Minghua.,Yang, Sijin.,Zhang, Dan.,Shui, Pixian.,Song, Shanshan.,...&Sun, Qin.(2014).Fructopyrano-(1 -> 4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE,7(11),3859-3869. |
| MLA | Liu, Minghua,et al."Fructopyrano-(1 -> 4)-glucopyranose inhibits the proliferation of liver cancer cells and angiogenesis in a VEGF/VEGFR dependent manner".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE 7.11(2014):3859-3869. |
入库方式： OAI收割
来源：上海药物研究所
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