Design, synthesis and properties of artificial nucleic acids from (R)-4-amino-butane-1,3-diol
文献类型：期刊论文
| 作者 | Li, Pengfei1,2; Sun, Jingjing1,2; Su, Meng1,2; Yang, Xiaogai3; Tang, Xinjing1,2 |
| 刊名 | ORGANIC & BIOMOLECULAR CHEMISTRY
 |
| 出版日期 | 2014 |
| 卷号 | 12期号:14页码:2263-2272 |
| ISSN号 | 1477-0520 |
| DOI | 10.1039/c3ob42291g |
| 文献子类 | Article |
| 英文摘要 | A new artificial nucleic acid analogue, (R)-Am-BuNA, was developed with a simplified acyclic (R)-4-amino-butane-1,3-diol phosphodiester backbone. Phosphoramidite monomers of (R)-Am-BuNA were incorporated into DNA oligonucleotides (ODNs) and G-quadruplexes. Their thermal stability, conformation change and biological stability were further investigated using UV-melting, circular dichroism (CD) and gel electrophoresis. The results suggested that thermal stability of the duplexes of (R)-Am-BuNA modified ODNs and their complementary ODN is highly dependent on the substitution position. Substitution of thymidine at the 7th position in a thrombin-binding DNA aptamer (TBA) results in a slight increase in T-m with no effect on quadruplex conformation on the CD spectrum in comparison to that of the natural G-quadruplex. Further enzymatic experiments with fetal bovine serum (FBS) and snake venom phosphodiesterase (SVPDE) indicated that only single replacement of a (R)-Am-BuNA modified nucleobase greatly inhibited oligonucleotide degradation, which shows their promising applications as capping nucleotides in nucleic acid drugs. |
| WOS关键词 | CHEMICAL-MODIFICATION ; ACYCLIC NUCLEOTIDE ; DUPLEX STABILITY ; GENETIC POLYMERS ; DNA APTAMER ; THROMBIN ; ANALOGS ; OLIGONUCLEOTIDES ; ANTISENSE ; SIRNAS |
| 资助项目 | National Basic Research Program of China (973 Program)[2012CB720600] ; National Natural Science Foundation of China[21072015] ; Program for New Century Excellent Talents in University[NCET-10-0203] ; State Key Laboratory of Drug Research[SIMM1106KF-15] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000333037500013 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277302]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Tang, Xinjing |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China; 3.Peking Univ, Sch Pharmaceut Sci, Dept Chem Biol, Beijing 100191, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Pengfei,Sun, Jingjing,Su, Meng,et al. Design, synthesis and properties of artificial nucleic acids from (R)-4-amino-butane-1,3-diol[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2014,12(14):2263-2272. |
| APA | Li, Pengfei,Sun, Jingjing,Su, Meng,Yang, Xiaogai,&Tang, Xinjing.(2014).Design, synthesis and properties of artificial nucleic acids from (R)-4-amino-butane-1,3-diol.ORGANIC & BIOMOLECULAR CHEMISTRY,12(14),2263-2272. |
| MLA | Li, Pengfei,et al."Design, synthesis and properties of artificial nucleic acids from (R)-4-amino-butane-1,3-diol".ORGANIC & BIOMOLECULAR CHEMISTRY 12.14(2014):2263-2272. |
入库方式： OAI收割
来源：上海药物研究所
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