Mechanism of the inhibition of the STAT3 signaling pathway by EGCG
文献类型：期刊论文
| 作者 | Wang, Yang4; Ren, Xuequn4; Deng, Chaoyang5; Yang, Lu5; Yan, Erfu6; Guo, Tao1; Li, Yanmin3; Xu, Marvin Xuejun2 |
| 刊名 | ONCOLOGY REPORTS
 |
| 出版日期 | 2013-12 |
| 卷号 | 30期号:6页码:2691-2696 |
| 关键词 | epigallocatechin-3-gallate signal transducer and activator of transcription 3 hepatocellular carcinoma molecular docking |
| ISSN号 | 1021-335X |
| DOI | 10.3892/or.2013.2743 |
| 文献子类 | Article |
| 英文摘要 | Signal transducer and activator of transcription 3 (STAT3) is an oncogene that promotes cell survival, proliferation, and motility. In the present study, we explored the mechanism involved in the inhibition by epigallocatechin-3-gallate (EGCG) of STAT3 signaling as detected by surface plasmon resonance (SPR)-binding assays and in silico docking. Stat3-binding assay indicated that EGCG significantly interrupted Stat3 peptide binding at micromolar concentrations, and the docking experiments indicated that EGCG had a strong interaction with Arg-609, one of the key residues in the STAT3 SH2 domain that contributes greatly to Stat3 and phosphorylated peptide binding. Following treatment of the hepatocellular carcinoma cell lines BEL-7402 and QGY-7703 with EGCG, in vitro, EGCG significantly suppressed cell proliferation as detected by MTT assay, induced apoptosis as detected by flow cytometry, dramatically lowered the expression levels of phosphorylated Stat3 proteins (p-Stat3) as determined by immunoblot detection, and inhibited the expression of multiple genes including Bcl-xL, c-Myc, VEGF and cyclin D1 as demonstrated by RT-PCR analysis. In conclusion, our research data indicate that the anticancer function of green tea results from the inhibition of the STAT3 signaling pathway by EGCG. |
| WOS关键词 | TEA POLYPHENOLS ; HEPATOCELLULAR-CARCINOMA ; MOLECULAR TARGETS ; CANCER ; ACTIVATION ; EPIGALLOCATECHIN-3-GALLATE ; APOPTOSIS ; FIBROBLASTS ; RECEPTOR ; LIGANDS |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000330226200021 |
| 出版者 | SPANDIDOS PUBL LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277344]  |
| 专题 | 药物制剂研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Xu, Marvin Xuejun |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Ctr Drug Delivery Syst, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Zhengzhou Univ, Basic Med Coll, Dept Pharmacol, Zhengzhou 450001, Henan, Peoples R China 3.Shangqiu Normal Coll, Dept Phys, Shangqiu 476000, Peoples R China; 4.Henan Univ, Huaihe Hosp, Kaifeng 475000, Peoples R China; 5.Shanghai McAry Biomed Technol Co Ltd, Pudong New Area, Zhangjiang Hitech Pk, Shanghai 201203, Peoples R China; 6.Tianjin Univ, Dept Pharm, Tianjin 300072, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Yang,Ren, Xuequn,Deng, Chaoyang,et al. Mechanism of the inhibition of the STAT3 signaling pathway by EGCG[J]. ONCOLOGY REPORTS,2013,30(6):2691-2696. |
| APA | Wang, Yang.,Ren, Xuequn.,Deng, Chaoyang.,Yang, Lu.,Yan, Erfu.,...&Xu, Marvin Xuejun.(2013).Mechanism of the inhibition of the STAT3 signaling pathway by EGCG.ONCOLOGY REPORTS,30(6),2691-2696. |
| MLA | Wang, Yang,et al."Mechanism of the inhibition of the STAT3 signaling pathway by EGCG".ONCOLOGY REPORTS 30.6(2013):2691-2696. |
入库方式： OAI收割
来源：上海药物研究所
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