Oligomannurarate sulfate inhibits CXCL12/SDF-1-mediated proliferation and invasion of human tumor cells in vitro
文献类型：期刊论文
| 作者 | Wen, Wei-wei; Xie, Shao; Xin, Xian-liang; Geng, Mei-yu ; Ding, Jian; Chen, Yi
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2013-12 |
| 卷号 | 34期号:12页码:1554-1559 |
| 关键词 | cancer anticancer drug oligosaccharide oligomannurarate sulfate JG6 CXCL12/CXCR4 axis surface plasmon resonance molecular docking |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2013.83 |
| 文献子类 | Article |
| 英文摘要 | Aim: JG6 is a novel marine-derived oligosaccharide that has shown to inhibit angiogenesis and tumor metastasis. In this study, we sought to identify the potential target responsible for the anti-cancer activity of JG6. Methods: Human liver cancer cell line Bel-7402 and human cervical cancer cell line HeLa were examined. CXCL12-stimulated cell proliferation and migration were determined using a CCK-8 kit and a transwell assay, respectively. Western blotting was performed to examine the changes in CXCL12/CXCR4 axis. Molecular docking and surface plasmon resonance (SPR) were performed to characterize the possible interaction between JG6 and the CXCL12/CXCR4 axis. Results: Treatment with CXCL12 potently stimulated the proliferation and migration in both Bel-7402 and HeLa cells. Co-treatment of the cells with JG6 (10, 50 and 100 mu g/mL) dose-dependently impeded the CXCL12-stimulated cell proliferation and migration. Furthermore, CXCL12 rapidly induced phosphorylation of AKT, ERK, FAK and Paxillin in Bel-7402 and HeLa cells, whereas pretreatment with JG6 dose-dependently inhibited the CXCL12-induced phosphorylation of these proteins. The SPR assay showed that JG6 bound to CXCL12 with a high affinity. In molecular docking study, JG6 appeared to interact with CXCL12 via multiple polar interactions, including 6 ionic bonds and 7 hydrogen bonds. Conclusion: Inhibition of the CXCL12/CXCR4 axis by JG6 may account for its anticancer activity. |
| WOS关键词 | HEPARAN-SULFATE ; GLYCOSAMINOGLYCAN ; ANGIOGENESIS ; MIGRATION ; DOCKING ; GROWTH ; CANCER ; GLIDE ; MODEL ; AXIS |
| 资助项目 | National Natural Science Foundation of China[81072666] ; National Basic Research Program Grant of China[2013CB932503] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:5003746 |
| WOS记录号 | WOS:000328268600009 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277352]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, Jian |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Wei-wei,Xie, Shao,Xin, Xian-liang,et al. Oligomannurarate sulfate inhibits CXCL12/SDF-1-mediated proliferation and invasion of human tumor cells in vitro[J]. ACTA PHARMACOLOGICA SINICA,2013,34(12):1554-1559. |
| APA | Wen, Wei-wei,Xie, Shao,Xin, Xian-liang,Geng, Mei-yu,Ding, Jian,&Chen, Yi.(2013).Oligomannurarate sulfate inhibits CXCL12/SDF-1-mediated proliferation and invasion of human tumor cells in vitro.ACTA PHARMACOLOGICA SINICA,34(12),1554-1559. |
| MLA | Wen, Wei-wei,et al."Oligomannurarate sulfate inhibits CXCL12/SDF-1-mediated proliferation and invasion of human tumor cells in vitro".ACTA PHARMACOLOGICA SINICA 34.12(2013):1554-1559. |
入库方式： OAI收割
来源：上海药物研究所
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