Berberine combined with 2-deoxy-D-glucose synergistically enhances cancer cell proliferation inhibition via energy depletion and unfolded protein response disruption
文献类型：期刊论文
| 作者 | Fan, Li-xia1; Liu, Chang-mei1; Gao, An-hui1,2; Zhou, Yu-bo2 ; Li, Jia1,2
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| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
 |
| 出版日期 | 2013-11 |
| 卷号 | 1830期号:11页码:5175-5183 |
| 关键词 | Berberine 2-Dem-D-glucose UPR GRP78 Cancer metabolism |
| ISSN号 | 0304-4165 |
| DOI | 10.1016/j.bbagen.2013.07.010 |
| 文献子类 | Article |
| 英文摘要 | Background: Targeting multiple aspects of cellular metabolism, such as both aerobic glycolysis and mitochondrial oxidative phosphorylation (OXPHOS), has the potential to improve cancer therapeutics. Berberine (BBR), a widely used traditional Chinese medicine, exerts its antitumor effects by inhibiting OXPHOS. 2-Deoxy-n-glucose (2-DG) targets aerobic glycolysis and demonstrates potential anticancer effects in the clinic. We hypothesized that BBR in combination with 2-DG would be more efficient than either agent alone against cancer cell growth. Methods: The effects of BBR and 2-DG on cancer cell growth were evaluated using the Sulforhodamine B (SRB) method. Cell death was detected with the PI uptake assay, and Western blot, Q-PCR and luciferase reporter assays were used for signaling pathway detection. An adenovirus system was used for gene overexpression. Results: BBR combined with 2-DG synergistically enhanced the growth inhibition of cancer cells in vitro. Further mechanistic studies showed that the combination drastically enhanced ATP depletion and strongly disrupted the unfolded protein response (UPR). Overexpressing GRP78 partially prevented the cancer cell inhibition induced by both compounds. Conclusions: Here, we report for the first time that BBR and 2-DG have a synergistic effect on cancer cell growth inhibition related to ATP energy depletion and disruption of UPR. General significance: Our results propose the potential use of BBR and 2-DG in combination as an anticancer treatment, reinforcing the hypothesis that targeting both aerobic glycolysis and OXPHOS provides more effective cancer therapy and highlighting the important role of UPR in the process. (C) 2013 Elsevier B.V. All rights reserved. |
| WOS关键词 | ENDOPLASMIC-RETICULUM STRESS ; IN-VITRO ; GLYCOLYTIC INHIBITOR ; PROSTATE-CANCER ; MELANOMA-CELLS ; BREAST-CANCER ; TUMOR-CELLS ; METABOLISM ; 2-DEOXYGLUCOSE ; APOPTOSIS |
| 资助项目 | National Natural Science Foundation of China[91029716] ; National Natural Science Foundation of China[81072667] ; National Natural Science Foundation of China[81021062] ; National Science and Technology Major Projects[2012ZX09301001-004] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Biophysics |
| 语种 | 英语 |
| WOS记录号 | WOS:000326207600022 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277404]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhou, Yu-bo |
| 作者单位 | 1.E China Normal Univ, Sch Life Sci, Shanghai 200237, Peoples R China; 2.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fan, Li-xia,Liu, Chang-mei,Gao, An-hui,et al. Berberine combined with 2-deoxy-D-glucose synergistically enhances cancer cell proliferation inhibition via energy depletion and unfolded protein response disruption[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2013,1830(11):5175-5183. |
| APA | Fan, Li-xia,Liu, Chang-mei,Gao, An-hui,Zhou, Yu-bo,&Li, Jia.(2013).Berberine combined with 2-deoxy-D-glucose synergistically enhances cancer cell proliferation inhibition via energy depletion and unfolded protein response disruption.BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,1830(11),5175-5183. |
| MLA | Fan, Li-xia,et al."Berberine combined with 2-deoxy-D-glucose synergistically enhances cancer cell proliferation inhibition via energy depletion and unfolded protein response disruption".BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS 1830.11(2013):5175-5183. |
入库方式： OAI收割
来源：上海药物研究所
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