中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 药物制剂研究中心
Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high performance affinity chromatography

文献类型：期刊论文

作者Li, Haiyan4; Ge, Jingwen4,5; Guo, Tao4; Yang, Shuo4,6; He, Zhonggui5; York, Peter1; Sun, Lixin2; Xu, Xu3; Zhang, Jiwen3,4
刊名JOURNAL OF CHROMATOGRAPHY A
出版日期2013-08-30
卷号1305页码:139-148
关键词Modified peak profiling method High performance affinity chromatography Cyclodextrin supramolecular systems Dissociation rate constant Plate height
ISSN号0021-9673
DOI10.1016/j.chroma.2013.07.010
文献子类Article
英文摘要It is challenging and extremely difficult to measure the kinetics of supramolecular systems with extensive, weak binding (K-a < 10(5) M-1), and fast dissociation, such as those composed of cyclodextrins and drugs. In this study, a modified peak profiling method based on high performance affinity chromatography (HPAC) was established to determine the dissociation rate constant of cyclodextrin supramolecular systems. The interactions of beta-cyclodextrin with acetaminophen and sertraline were used to exemplify the method. The retention times, variances and the plate heights of the peaks for acetaminophen or sertraline, conventional non-retained substance (H2O) on the beta-cyclodextrin bonded column and a control column were determined at four flow rates under linear elution conditions. Then, plate heights for the theoretical non-retained substance were estimated by the modified HPAC method, in consideration of the diffusion and stagnant mobile phase mass transfer. As a result, apparent dissociation rate constants of 1.82 (+/- 0.01)s(-1) and 3.55 (+/- 0.37)s(-1) were estimated for acetaminophen and sertraline respectively at pH 6.8 and 25 degrees C with multiple flow rates. Following subtraction of the non-specific binding with the support, dissociation rate constants were estimated as 1.78 (+/- 0.00) and 1.91 (+/- 0.02)s(-1) for acetaminophen and sertraline, respectively. These results for acetaminophen and sertraline were in good agreement with the magnitude of the rate constants for other drugs determined by capillary electrophoresis reported in the literature and the peak fitting method we performed. The method described in this work is thought to be suitable for other supramolecules, with relatively weak, fast and extensive interactions. (C) 2013 Elsevier B.V. All rights reserved.
WOS关键词HUMAN SERUM-ALBUMIN ; DRUG-PROTEIN DISSOCIATION ; FLUORESCENCE CORRELATION SPECTROSCOPY ; PEAK DECAY ANALYSIS ; VOID DEAD VOLUME ; BETA-CYCLODEXTRIN ; BINDING-KINETICS ; LIQUID-CHROMATOGRAPHY ; BIOLOGICAL-MEMBRANES ; STATIONARY-PHASE
资助项目National Natural Science Foundation of China[81102389] ; International Science and Technology Cooperation Project[S2010GR0920] ; National Science and Technology Major Project of China[2012ZX09301001-001]
WOS研究方向Biochemistry & Molecular Biology ; Chemistry
语种英语
WOS记录号WOS:000323362200018
出版者ELSEVIER SCIENCE BV
源URL[http://119.78.100.183/handle/2S10ELR8/277495]  
专题药物制剂研究中心
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Zhang, Jiwen
作者单位1.Univ Bradford, Bradford BD7 1DP, W Yorkshire, England;
2.Shenyang Pharmaceut Univ, Sch Pharm, Dept Pharmaceut Anal, Shenyang 110016, Peoples R China;
3.Shanghai Inst Technol, Sch Chem & Environm Engn, Shanghai 201418, Peoples R China
4.Chinese Acad Sci, Ctr Drug Delivery Syst, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
5.Shenyang Pharmaceut Univ, Sch Pharm, Shenyang 110016, Peoples R China;
6.Guizhou Univ, Guiyang 550025, Peoples R China;
推荐引用方式
GB/T 7714		 Li, Haiyan,Ge, Jingwen,Guo, Tao,et al. Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high performance affinity chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1305:139-148.
APA Li, Haiyan.,Ge, Jingwen.,Guo, Tao.,Yang, Shuo.,He, Zhonggui.,...&Zhang, Jiwen.(2013).Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high performance affinity chromatography.JOURNAL OF CHROMATOGRAPHY A,1305,139-148.
MLA Li, Haiyan,et al."Determination of the kinetic rate constant of cyclodextrin supramolecular systems by high performance affinity chromatography".JOURNAL OF CHROMATOGRAPHY A 1305(2013):139-148.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。