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Multi-Drug-Resistant Cells Enriched From Chronic Myeloid Leukemia Cells by Doxorubicin Possess Tumor-Initiating-Cell Properties

文献类型：期刊论文

作者Xin, Hong1; Kong, Ying1; Jiang, Xiaoxiao2; Wang, Ke3; Qin, Xiaoran3; Miao, Ze-Hong4; Zhu, Yizhun1; Tan, Wenfu1,4
刊名JOURNAL OF PHARMACOLOGICAL SCIENCES
出版日期2013-08
卷号122期号:4页码:299-304
关键词multi-drug resistance K562/A02 tumor-initiating cell snail twist1
ISSN号1347-8613
DOI10.1254/jphs.13025FP
文献子类Article
英文摘要Multiple drug resistance (MDR) occurring during chemotherapy is a major obstacle for treatment of cancers using chemotherapeutic drugs; thus, the mechanisms underlying MDR have attracted intensive attention. Many studies have shown that tumor-initiating cells exhibit a chemotherapeutic tolerance characteristic. However, whether the MDR cells possess tumor-initiating cells properties and its underlying mechanisms remain to be fully elucidated. In this study, we utilized a well-established MDR cell line K562/A02 enriched by doxorubicin from K562 cells to determine if the K562/A02 cells possess tumor-initiating properties and investigated its potential molecular mechanisms. We observed that the expressions of Oct4, Sox2, and Nanog, all of which are well-characterized stem cell markers, in K562/A02 cells were elevated in comparison to parental K562 cells; in addition, we found that K562/A02 cells exhibited more potent in vitro and in vivo tumor-initiating properties, as revealed by sphere assay, self-renewal assay, soft agar assay, and animal studies. Furthermore, our data suggest that snail and twist 1, two well known transcriptional factors for the epithelial-mesenchymal transition (EMT) program, may be potentially involved in the acquisition of tumor-initiating properties of K562/A02 cells. Thus, our study demonstrates that MDR K562/A02 cells possess tumor-initiating properties, most likely due to the elevated expressions of snail and twist1
WOS关键词CANCER STEM-CELLS ; IDENTIFICATION ; MECHANISMS
资助项目National Natural Science Foundation of China[81173077] ; "Interdisciplinary Cooperation Team" Program for Science and Technology Innovation of the Chinese Academy of Sciences[00000000] ; State Key Laboratory of Drug Research of Chinese Academy of Sciences[SIMM1004KF-07]
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000323586300008
出版者JAPANESE PHARMACOLOGICAL SOC
源URL[http://119.78.100.183/handle/2S10ELR8/277516]  
专题药理学第一研究室
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Zhu, Yizhun
作者单位1.Fudan Univ, Sch Pharm, Dept Pharmacol, Shanghai 201203, Peoples R China;
2.Shandong Univ Weihai, Dept Pharmacol, Weihai 264209, Peoples R China;
3.Shanghai ChemPartner Co Ltd, Dept Pharmacol, Shanghai 201203, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
推荐引用方式
GB/T 7714		 Xin, Hong,Kong, Ying,Jiang, Xiaoxiao,et al. Multi-Drug-Resistant Cells Enriched From Chronic Myeloid Leukemia Cells by Doxorubicin Possess Tumor-Initiating-Cell Properties[J]. JOURNAL OF PHARMACOLOGICAL SCIENCES,2013,122(4):299-304.
APA Xin, Hong.,Kong, Ying.,Jiang, Xiaoxiao.,Wang, Ke.,Qin, Xiaoran.,...&Tan, Wenfu.(2013).Multi-Drug-Resistant Cells Enriched From Chronic Myeloid Leukemia Cells by Doxorubicin Possess Tumor-Initiating-Cell Properties.JOURNAL OF PHARMACOLOGICAL SCIENCES,122(4),299-304.
MLA Xin, Hong,et al."Multi-Drug-Resistant Cells Enriched From Chronic Myeloid Leukemia Cells by Doxorubicin Possess Tumor-Initiating-Cell Properties".JOURNAL OF PHARMACOLOGICAL SCIENCES 122.4(2013):299-304.

入库方式： OAI收割

来源：上海药物研究所

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