Targeted editing of goat genome with modular-assembly zinc finger nucleases based on activity prediction by computational molecular modeling
文献类型：期刊论文
| 作者 | Xiong, Kai2; Li, Shanshan3,4; Zhang, Hongxiao2; Cui, Ye1; Yu, Debing2; Li, Yan2; Sun, Wenxing2; Fu, Yingying2; Teng, Yun2; Liu, Zhi2 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2013-07 |
| 卷号 | 40期号:7页码:4251-4256 |
| 关键词 | ZFN Genome editing Modular-assembly BLG Molecular modeling Goat |
| ISSN号 | 0301-4851 |
| DOI | 10.1007/s11033-013-2507-5 |
| 文献子类 | Article |
| 英文摘要 | Zinc finger nuclease (ZFN) technology can mediate targeted genome modification to produce transgenic animals in a high-efficient and biological-safe way. Modular assembly is a rapid, convenient and open-source method for the synthesis of ZFNs. However, this biotechnology is hampered by multistep construction, low-efficiency editing and off-target cleavage. Here we synthesized and tested six pairs of three- or four-finger ZFNs to target one site in goat beta-lactoglobulin (BLG, a dominant allergen in goat milk) gene. Homology modeling was applied to build the structure model of ZFNs to predict their editing activities targeting at goat BLG gene. Goat fibroblast cells were transfected with plasmids that encoded ZFN pairs, and genomic DNA was isolated 72 h later for genome editing efficiency assay. The results of editing efficiency assay demonstrated that ZFNs with optimal interaction modes can edit goat BLG gene more efficiently, whereas ZFNs with unexpected interaction modes showed lower activities in editing BLG gene. We concluded that modular-assembly ZFNs can provide a rapid, public-available, and easy-to-practice platform for transgenic animal research and molecular modeling would help as a useful tool for ZFNs activity prediction. |
| WOS关键词 | TRANSCRIPTION FACTORS ; RECOGNITION CODE ; DNA-SEQUENCES ; SOMATIC-CELLS ; GENE ; DROSOPHILA ; DOMAINS ; PROTEIN ; CLEAVAGE ; FAMILY |
| 资助项目 | China National Program for Transgenic Animal[2011ZX08008-004] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000320674700015 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277567]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Chen, Jie |
| 作者单位 | 1.China Pharmaceut Univ, Ctr Drug Discovery, Nanjing 210009, Jiangsu, Peoples R China 2.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Jiangsu, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Medica, Drug Discovery & Design Ctr, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 4.China Pharmaceut Univ, Sch Sci, Lab Mol Design & Drug Discovery, Nanjing 210009, Jiangsu, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xiong, Kai,Li, Shanshan,Zhang, Hongxiao,et al. Targeted editing of goat genome with modular-assembly zinc finger nucleases based on activity prediction by computational molecular modeling[J]. MOLECULAR BIOLOGY REPORTS,2013,40(7):4251-4256. |
| APA | Xiong, Kai.,Li, Shanshan.,Zhang, Hongxiao.,Cui, Ye.,Yu, Debing.,...&Chen, Jie.(2013).Targeted editing of goat genome with modular-assembly zinc finger nucleases based on activity prediction by computational molecular modeling.MOLECULAR BIOLOGY REPORTS,40(7),4251-4256. |
| MLA | Xiong, Kai,et al."Targeted editing of goat genome with modular-assembly zinc finger nucleases based on activity prediction by computational molecular modeling".MOLECULAR BIOLOGY REPORTS 40.7(2013):4251-4256. |
入库方式： OAI收割
来源：上海药物研究所
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