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The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus

文献类型：期刊论文

作者Wang, Jun2; Wang, Yu-hua2,3; Hou, Yuan-yuan1; Xi, Tao2; Liu, Yao1; Liu, Jing-gen1
刊名ACTA PHARMACOLOGICA SINICA
出版日期2013-06
卷号34期号:6页码:811-818
关键词morphine withdrawal aversive memory conditioned place aversion dorsal hippocampus actin rearrangement RhoA Rac1 naloxone Y27632 NSC23766
ISSN号1671-4083
DOI10.1038/aps.2013.3
文献子类Article
英文摘要Aim: Actin rearrangements are induced in the dorsal hippocampus after conditioned morphine withdrawal, and involved in the formation of conditioned place aversion. In the present study, we investigated the mechanisms underlying the actin rearrangements in rat dorsal hippocampus induced by conditioned morphine withdrawal. Methods: The RhoA-ROCK pathway inhibitor Y27632 (8.56 mu g/1 mu L per side) or the Rac1 inhibitor NSC23766 (25 mu g/1 mu L per side) was microinjected into the dorsal hippocampus of rats. Conditioned place aversion (CPA) induced by naloxone-precipitated morphine withdrawal was assessed. Crude synaptosomal fraction of hippocampus was prepared, and the amount of F-actin and G-actin was measured with an Actin Polymerization Assay Kit. Results: Conditioned morphine withdrawal significantly increased actin polymerization in the dorsal hippocampus at 1 h following the naloxone injection. Preconditioning with microinjection of Y27632, but not NSC23766, attenuated CPA, and blocked the increase in actin polymerization in the dorsal hippocampus. Conclusion: Our results suggest that the small GTPase RhoA, but not Rac1, in the dorsal hippocampus is responsible for CPA formation, mainly through its regulation of actin rearrangements.
WOS关键词TERM SYNAPTIC PLASTICITY ; DENDRITIC SPINES ; STRUCTURAL PLASTICITY ; DRUG-WITHDRAWAL ; CYTOSKELETON ; DYNAMICS ; FAMILY ; POLYMERIZATION ; MAINTENANCE ; EXPRESSION
资助项目Ministry of Science and Technology of China[2009CB522005] ; Foundation of National Natural Science of China[81130087] ; Foundation of National Natural Science of China[81001424] ; Foundation of Natural Science of Jiangshu Province[SBK201241521]
WOS研究方向Chemistry ; Pharmacology & Pharmacy
语种英语
CSCD记录号CSCD:4850358
WOS记录号WOS:000319915000013
出版者ACTA PHARMACOLOGICA SINICA
源URL[http://119.78.100.183/handle/2S10ELR8/277597]  
专题药理学第二研究室
中科院受体结构与功能重点实验室
新药研究国家重点实验室
通讯作者Xi, Tao
作者单位1.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
2.China Pharmaceut Univ, Sch Life Sci & Technol, Ctr Biotechnol, Nanjing 210009, Jiangsu, Peoples R China;
3.Nanjing Univ Chinese Med, Sch Pharm, Nanjing 210046, Jiangsu, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Jun,Wang, Yu-hua,Hou, Yuan-yuan,et al. The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus[J]. ACTA PHARMACOLOGICA SINICA,2013,34(6):811-818.
APA Wang, Jun,Wang, Yu-hua,Hou, Yuan-yuan,Xi, Tao,Liu, Yao,&Liu, Jing-gen.(2013).The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus.ACTA PHARMACOLOGICA SINICA,34(6),811-818.
MLA Wang, Jun,et al."The small GTPase RhoA, but not Rac1, is essential for conditioned aversive memory formation through regulation of actin rearrangements in rat dorsal hippocampus".ACTA PHARMACOLOGICA SINICA 34.6(2013):811-818.

入库方式： OAI收割

来源：上海药物研究所

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