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Oxidation-sensing Regulator AbfR Regulates Oxidative Stress Responses, Bacterial Aggregation, and Biofilm Formation in Staphylococcus epidermidis

文献类型：期刊论文

作者Liu, Xing1; Sun, Xiaoxu1; Wu, Youcong2,3,4; Xie, Cen1; Zhang, Wenru1; Wang, Dan5,6; Chen, Xiaoyan1; Qu, Di2,3,4; Gan, Jianhua7; Chen, Hao5,6
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2013-02-08
卷号288期号:6页码:3739-3752
ISSN号0021-9258
DOI10.1074/jbc.M112.426205
文献子类Article
英文摘要Staphylococcus epidermidis is a notorious human pathogen that is the major cause of infections related to implanted medical devices. Although redox regulation involving reactive oxygen species is now recognized as a critical component of bacterial signaling and regulation, the mechanism by which S. epidermidis senses and responds to oxidative stress remains largely unknown. Here, we report a new oxidation-sensing regulator, AbfR (aggregation and biofilm formation regulator) in S. epidermidis. An environment of oxidative stress mediated by H2O2 or cumene hydroperoxide markedly up-regulates the expression of abfR gene. Similar to Pseudomonas aeruginosa OspR, AbfR is negatively autoregulated and dissociates from promoter DNA in the presence of oxidants. In vivo and in vitro analyses indicate that Cys-13 and Cys-116 are the key functional residues to form an intersubunit disulfide bond upon oxidation in AbfR. We further show that deletion of abfR leads to a significant induction in H2O2 or cumene hydroperoxide resistance, enhanced bacterial aggregation, and reduced biofilm formation. These effects are mediated by derepression of SERP2195 and gpxA-2 that lie immediately downstream of the abfR gene in the same operon. Thus, oxidative stress likely acts as a signal to modulate S. epidermidis key virulence properties through AbfR.
WOS关键词FAMILY TRANSCRIPTIONAL REGULATORS ; DISULFIDE BOND FORMATION ; WALL TEICHOIC-ACIDS ; ANTIBIOTIC-RESISTANCE ; GLOBAL REGULATOR ; REDOX-SWITCH ; MARR FAMILY ; ORGANIC HYDROPEROXIDES ; CRYSTAL-STRUCTURES ; HYDROGEN-PEROXIDE
资助项目National Natural Science Foundation of China[90913010] ; National Natural Science Foundation of China[21172234] ; National Natural Science Foundation of China[20972173] ; National Natural Science Foundation of China[81271791] ; Hundred Talent Program of the Chinese Academy of Sciences[00000000] ; National Science and Technology Key New Drug Creation and Manufacturing Program Major Project[2013ZX09507-004] ; Natural Science Foundation of Jiangsu Province[BK2010358]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000314845000006
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
源URL[http://119.78.100.183/handle/2S10ELR8/277733]  
专题药理学第三研究室
中科院受体结构与功能重点实验室
新药研究国家重点实验室
分析化学研究室
通讯作者Lan, Lefu
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China;
2.Fudan Univ, Minist Educ, Key Lab Med Mol Virol, Shanghai 200032, Peoples R China;
3.Fudan Univ, Minist Hlth, Inst Med Microbiol, Shanghai 200032, Peoples R China;
4.Fudan Univ, Inst Biomed Sci, Shanghai Med Sch, Shanghai 200032, Peoples R China;
5.Nanjing Univ, State Key Lab Coordinat Chem, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China;
6.Nanjing Univ, Inst Coordinat Chem, Sch Chem & Chem Engn, Nanjing 210093, Jiangsu, Peoples R China;
7.Fudan Univ, Sch Life Sci, Shanghai 200433, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Xing,Sun, Xiaoxu,Wu, Youcong,et al. Oxidation-sensing Regulator AbfR Regulates Oxidative Stress Responses, Bacterial Aggregation, and Biofilm Formation in Staphylococcus epidermidis[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2013,288(6):3739-3752.
APA Liu, Xing.,Sun, Xiaoxu.,Wu, Youcong.,Xie, Cen.,Zhang, Wenru.,...&Yang, Cai-Guang.(2013).Oxidation-sensing Regulator AbfR Regulates Oxidative Stress Responses, Bacterial Aggregation, and Biofilm Formation in Staphylococcus epidermidis.JOURNAL OF BIOLOGICAL CHEMISTRY,288(6),3739-3752.
MLA Liu, Xing,et al."Oxidation-sensing Regulator AbfR Regulates Oxidative Stress Responses, Bacterial Aggregation, and Biofilm Formation in Staphylococcus epidermidis".JOURNAL OF BIOLOGICAL CHEMISTRY 288.6(2013):3739-3752.

入库方式： OAI收割

来源：上海药物研究所

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