Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography
文献类型：期刊论文
| 作者 | He, Minfei1; Du, Wenkai1; Du, Qingbao1; Zhang, Yun1; Li, Bo1; Ke, Changqian2; Ye, Yang2 ; Du, Qizhen1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2013-01-04 |
| 卷号 | 1271页码:67-70 |
| 关键词 | All-trans-retinal cis-Retinal isomer Flash countercurrent chromatography |
| ISSN号 | 0021-9673 |
| DOI | 10.1016/j.chroma.2012.11.022 |
| 文献子类 | Article; Proceedings Paper |
| 英文摘要 | The isolation of the retinal isomers from all-trans-retinal was performed by flash countercurrent chromatography. In each separation, isomerization reaction solution of 200 mg all-trans-retinal could be loaded on a 1200 mL of high-speed countercurrent chromatographic column with 5 mm bore, eluted by a mobile phase flow rate of 25 mL/min, resulting in 63 mg of 11-cis-retinal, 24 mg of 13-cis-retinal and 26 mg of 9-cis-retinal with purities more than 95%. n-Hexane-acetonitrile (3:1) was used as the solvent system which possesses the advantages of simplicity, re-use of the solvent and multiple injections. This method could be used to prepare 13-cis-retinal, 11-cis-retinal and 9-cis-retinal for the photoisomerization investigation, such as the effect of 11-cis-retinal in the visual system. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS关键词 | VISUAL PIGMENTS ; RHODOPSIN |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000314016500010 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277770]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ye, Yang |
| 作者单位 | 1.Zhejiang Gongshang Univ, Inst Food Chem, Hangzhou 3100125, Zhejiang, Peoples R China; 2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Minfei,Du, Wenkai,Du, Qingbao,et al. Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography[J]. JOURNAL OF CHROMATOGRAPHY A,2013,1271:67-70. |
| APA | He, Minfei.,Du, Wenkai.,Du, Qingbao.,Zhang, Yun.,Li, Bo.,...&Du, Qizhen.(2013).Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography.JOURNAL OF CHROMATOGRAPHY A,1271,67-70. |
| MLA | He, Minfei,et al."Isolation of the retinal isomers from the isomerization of all-trans-retinal by flash countercurrent chromatography".JOURNAL OF CHROMATOGRAPHY A 1271(2013):67-70. |
入库方式： OAI收割
来源：上海药物研究所
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