Label-free monitoring of T cell activation by the impedance-based xCELLigence system
文献类型：期刊论文
| 作者 | Guan, Ni1; Deng, Jiejie1,2; Li, Ting1; Xu, Xiao1,3; Irelan, Jeffrey T.3; Wang, Ming-Wei1,2
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| 刊名 | MOLECULAR BIOSYSTEMS
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| 出版日期 | 2013 |
| 卷号 | 9期号:5页码:1035-1043 |
| ISSN号 | 1742-206X |
| DOI | 10.1039/c3mb25421f |
| 文献子类 | Article |
| 英文摘要 | T cells play a critical role in maintaining the normal function of the adaptive immune response, with their dysfunction resulting in a variety of autoimmune and immunodeficiency diseases. Efficient and accurate detection of T cell function is therefore crucial to clinical diagnosis and development of immunomodulators. A variety of in vitro cellular systems are currently employed for analyzing T cell activation, yet all suffer from some combination of low throughput, unnatural conditions and long assay times. Label-free technologies are capable of detecting phenotypic responses to treatments under physiological conditions, thereby potentially accelerating drug discovery by facilitating the use of disease-relevant cell models for functional assessment and clinical diagnosis. The xCELLigence system is an impedance based label-free platform that allows for dynamic monitoring of subtle morphological and adhesive changes in cells, such as those induced during T cell activation. Here we describe the development and validation of a T cell activation assay based upon electrical impedance. Co-activation of Jurkat cells with anti-CD28 and anti-CD3 functional antibodies led to impedance changes that were rapidly and sensitively recorded (within 30 minutes). This phenomenon was also observed in human peripheral blood mononuclear cells. These changes reflect morphological and adhesive alterations correlated with cytoskeletal reorganization as verified by microscopy. They were functionally dependent on canonical T cell signaling pathways, including calcium-mediated signals and Src family kinases because relevant inhibitors impaired T cell activation. Our results provide a convenient approach to measure T cell activation in real-time and to elucidate the underlying mechanisms of action through probing with small molecules. |
| WOS关键词 | PROLINE-RICH SEQUENCE ; TRANSCRIPTIONAL REGULATION ; LYMPHOCYTE DIVISION ; ACTIN CYTOSKELETON ; IMMUNE-RESPONSES ; ANTIGEN RECEPTOR ; FLOW-CYTOMETRY ; SENSOR ARRAYS ; CALCIUM ; TCR |
| 资助项目 | Ministry of Health of China[2012ZX09304-011] ; Ministry of Health of China[2013ZX09507002] ; Shanghai Science and Technology Development Fund[11DZ2292200] ; CAS-Novo Nordisk Research Fund[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000316963500025 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277801]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wang, Ming-Wei |
| 作者单位 | 1.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 3.Hangzhou High Throughput Drug Screening Ctr, Hangzhou 310030, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Ni,Deng, Jiejie,Li, Ting,et al. Label-free monitoring of T cell activation by the impedance-based xCELLigence system[J]. MOLECULAR BIOSYSTEMS,2013,9(5):1035-1043. |
| APA | Guan, Ni,Deng, Jiejie,Li, Ting,Xu, Xiao,Irelan, Jeffrey T.,&Wang, Ming-Wei.(2013).Label-free monitoring of T cell activation by the impedance-based xCELLigence system.MOLECULAR BIOSYSTEMS,9(5),1035-1043. |
| MLA | Guan, Ni,et al."Label-free monitoring of T cell activation by the impedance-based xCELLigence system".MOLECULAR BIOSYSTEMS 9.5(2013):1035-1043. |
入库方式： OAI收割
来源：上海药物研究所
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