Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance
文献类型：期刊论文
| 作者 | Zhang, Xiaozhe1; Wang, Jingjing2; Feng, Yue1; Ge, Jingpeng1; Li, Wenfei1; Sun, Wending2; Iscla, Irene3; Yu, Jie1; Blount, Paul3; Li, Yang2
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| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2012-10-30 |
| 卷号 | 109期号:44页码:18180-18185 |
| 关键词 | crystal structure anion selection cytoplasmic region electrophysiology single channel recording |
| ISSN号 | 0027-8424 |
| DOI | 10.1073/pnas.1207977109 |
| 文献子类 | Article |
| 英文摘要 | Mechanosensitive (MS) channels are universal cellular membrane pores. Bacterial MS channels, as typified by MS channel of small conductance (MscS) from Escherichia coli (EcMscS), release osmolytes under hypoosmotic conditions. MS channels are known to be ion selective to different extents, but the underlying mechanism remains poorly understood. Here we identify an anion-selective MscS channel from Thermoanaerobacter tengcongensis (TtMscS). The structure of TtMscS closely resembles that of EcMscS, but it lacks the large cytoplasmic equatorial portals found in EcMscS. In contrast, the cytoplasmic pore formed by the C-terminal beta-barrel of TtMscS is larger than that of EcMscS and has a strikingly different pattern of electrostatic surface potential. Swapping the beta-barrel region between TtMscS and EcMscS partially switches the ion selectivity. Our study defines the role of the beta-barrel in the ion selection of an anion-selective MscS channel and provides a structural basis for understanding the ion selectivity of MscS channels. |
| WOS关键词 | ESCHERICHIA-COLI MSCS ; ION CHANNELS ; CYTOPLASMIC DOMAIN ; CRYSTAL-STRUCTURE ; MECHANOTRANSDUCTION ; PERMEATION ; MUTATIONS ; MICROBES ; BILAYERS ; RECEPTOR |
| 资助项目 | Ministry of Science and Technology[2011CB910502] ; Ministry of Science and Technology[2012CB911101] ; National Natural Science Foundation[31030020] ; National Natural Science Foundation[31170679] ; National Natural Science Foundation[31171011] ; Chinese Academy of Sciences Foundation[2320103112110201] ; Cancer Prevention and Research Institute of Texas[RP100146] ; Welch Foundation[I-1420] ; National Institutes of Health[GM61028] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000311149900097 |
| 出版者 | NATL ACAD SCIENCES |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277904]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第二研究室 |
| 通讯作者 | Li, Yang |
| 作者单位 | 1.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, Sch Life Sci, Key Lab Prot Sci,Minist Educ, Beijing 100084, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Univ Texas SW Med Ctr Dallas, Dept Physiol, Dallas, TX 75390 USA; |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaozhe,Wang, Jingjing,Feng, Yue,et al. Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2012,109(44):18180-18185. |
| APA | Zhang, Xiaozhe.,Wang, Jingjing.,Feng, Yue.,Ge, Jingpeng.,Li, Wenfei.,...&Yang, Maojun.(2012).Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,109(44),18180-18185. |
| MLA | Zhang, Xiaozhe,et al."Structure and molecular mechanism of an anion-selective mechanosensitive channel of small conductance".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109.44(2012):18180-18185. |
入库方式： OAI收割
来源：上海药物研究所
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