Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells
文献类型：期刊论文
| 作者 | Ma, Yu6,7,8; Gu, Junjie6,7,8; Li, Chunliang1,6,7; Wei, Xiaoyuan2; Tang, Fan6,7,8; Shi, Guilai6,7; Jiang, Jing; Kuang, Ying; Li, Jinsong4; Wang, Zhugang3 |
| 刊名 | STEM CELL RESEARCH & THERAPY
 |
| 出版日期 | 2012-07-31 |
| 卷号 | 3 |
| ISSN号 | 1757-6512 |
| DOI | 10.1186/scrt120 |
| 文献子类 | Article |
| 英文摘要 | Introduction: Embryonic stem cells (ESCs) provide an attractive cell source for basic research and disease treatment. Currently, the common culture system for mouse ESC requires mouse embryonic fibroblast (MEF) as a feeder layer supplemented with leukemia inhibitory factor (LIF). The drawbacks associated with MEF and the cost of LIF have motivated exploration of new feeder cell types to maintain self-renewal of mouse ESCs without the need of exogenous LIF. However, why these feeder cells could maintain ESCs at the undifferentiated state independent of exogenous LIF is unclear. Methods: We derived mouse ESC lines using human foreskin fibroblast (HFF) in the absence of exogenous LIF. We also examined the dependence of HFF on the JAK-Stat3 pathway to maintain ESC identities and explored the potential molecular basis for HFF to support self-renewal of ESCs. Results: HFF supported mouse ESC self-renewal superiorly to MEFs. Using the HFF system, multiple lines of mouse ESCs were successfully derived without addition of exogenous LIF and any small molecular inhibitors. These ESCs had capacities to self-renew for a long period of time and to differentiate into various cell types of the three germ layers both in vitro and in vivo. Moreover, the ESCs participated in embryonic development and contributed to germ cell lineages in the chimeric mouse. At a molecular level, HFF was dependent on the JAK-Stat3 pathway to maintain ESC self-renewal. The high level of interleukin-6 (IL-6) produced by HFF might be responsible for the exogenous LIF-independent effect. Conclusion: This study describes an efficient, convenient and economic system to establish and maintain mouse ESC lines, and provides insights into the functional difference in the support of ESC culture between MEF and HFF. |
| WOS关键词 | LEUKEMIA INHIBITORY FACTOR ; UNDIFFERENTIATED GROWTH ; DIFFERENTIATION ; FEEDER ; STAT3 ; ESTABLISHMENT ; PLURIPOTENCY ; ACTIVATION ; CULTURE ; SYSTEM |
| 资助项目 | National Natural Science Foundation[91019023] ; National High Technology Research and Development Program of China[2010CB945200] ; National High Technology Research and Development Program of China[2011DFB300100] ; National High Technology Research and Development Program of China[2011CB965101] ; National High Technology Research and Development Program of China[2009CB941103] ; Chinese Academy of Science[XDA01010102] ; Shanghai Leading Academic Discipline Project[S30201] ; Ministry of Education of China[20110073110082] |
| WOS研究方向 | Cell Biology ; Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000308465000001 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278008]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Jin, Ying |
| 作者单位 | 1.St Jude Childrens Res Hosp, Dept Tumor Cell Biol, Memphis, TN 38103 USA; 2.Tongji Univ, Shanghai Key Lab Signaling & Dis Res, Sch Life Sci & Technol, Lab Receptor based BioMed, Shanghai 200092, Peoples R China; 3.Shanghai Res Ctr Model Organisms, Shanghai 201203, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China 6.Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200025, Peoples R China; 7.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200025, Peoples R China; 8.Shanghai Jiao Tong Univ, Sch Med, Shanghai Stem Cell Inst, Shanghai 200025, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Ma, Yu,Gu, Junjie,Li, Chunliang,et al. Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells[J]. STEM CELL RESEARCH & THERAPY,2012,3. |
| APA | Ma, Yu.,Gu, Junjie.,Li, Chunliang.,Wei, Xiaoyuan.,Tang, Fan.,...&Jin, Ying.(2012).Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells.STEM CELL RESEARCH & THERAPY,3. |
| MLA | Ma, Yu,et al."Human foreskin fibroblast produces interleukin-6 to support derivation and self-renewal of mouse embryonic stem cells".STEM CELL RESEARCH & THERAPY 3(2012). |
入库方式： OAI收割
来源：上海药物研究所
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