Chemical and biochemical approaches in the study of histone methylation and demethylation
文献类型：期刊论文
| 作者 | Li, Keqin Kathy1,4; Luo, Cheng2 ; Wang, Dongxia5; Jiang, Hualiang2; Zheng, Y. George3
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| 刊名 | MEDICINAL RESEARCH REVIEWS
 |
| 出版日期 | 2012-07 |
| 卷号 | 32期号:4页码:815-867 |
| 关键词 | histone methylation demethylation ChIP mass spectrometry chemical ligation inhibitor |
| ISSN号 | 0198-6325 |
| DOI | 10.1002/med.20228 |
| 文献子类 | Review |
| 英文摘要 | Histone methylation represents one of the most critical epigenetic events in DNA function regulation in eukaryotic organisms. Classic molecular biology and genetics tools provide significant knowledge about mechanisms and physiological roles of histone methyltransferases and demethylases in various cellular processes. In addition to this stream line, development and application of chemistry and chemistry-related techniques are increasingly involved in biological study, and offer information otherwise difficult to obtain by standard molecular biology methods. Herein, we review recent achievements and progress in developing and applying chemical and biochemical approaches in the study of histone methylation, including chromatin immunoprecipitation, chemical ligation, mass spectrometry, biochemical methylation and demethylation assays, and inhibitor development. These technological advances allow histone methylation to be studied from genome-wide level to molecular and atomic levels. With ChIP technology, information can be obtained about precise mapping of histone methylation patterns at specific promoters, genes, or other genomic regions. MS is particularly useful in detecting and analyzing methylation marks in histone and nonhistone protein substrates. Chemical approaches that permit site-specific incorporation of methyl groups into histone proteins greatly facilitate the investigation of biological impacts of methylation at individual modification sites. Discovery and design of selective organic inhibitors of histone methyltransferases and demethylases provide chemical probes to interrogate methylation-mediated cellular pathways. Overall, these chemistry-related technological advances have greatly improved our understanding of the biological functions of histone methylation in normal physiology and diseased states, and also are of great potential to translate basic epigenetics research into diagnostic and therapeutic applications in the clinic. (C) 2010 Wiley Periodicals, Inc. Med Res Rev., 32, No. 4, 815-867, 2012 |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; PROTEIN-ARGININE-METHYLTRANSFERASE ; ADENOSYLMETHIONINE-DEPENDENT METHYLTRANSFERASES ; TIME-OF-FLIGHT ; LYSINE 36-SPECIFIC METHYLTRANSFERASE ; CONTINUOUS SPECTROPHOTOMETRIC ASSAY ; ELECTRON-CAPTURE DISSOCIATION ; MECHANISM-BASED INACTIVATOR ; EMBRYONIC STEM-CELLS ; POSTTRANSLATIONAL MODIFICATIONS |
| 资助项目 | National Natural Science Foundation of China[20972174] ; Shanghai Science and Technology Innovation Program[10410703900] ; State Key Program of Basic Research of China[2009CB918502] ; National ST Major Project[2009ZX09501-001] ; Georgia Cancer Coalition Distinguished Cancer Scientist program[00000000] ; American Heart Association[00000000] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000306140000006 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278026]  |
| 专题 | 药物发现与设计中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zheng, Y. George |
| 作者单位 | 1.US SINO Epigenet Assoc, Norcross, GA USA; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Drug Discovery & Design Ctr, Shanghai 200031, Peoples R China; 3.Georgia State Univ, Dept Chem, Atlanta, GA 30302 USA; 4.Shanghai Jiao Tong Univ, Sch Med, Rui Jin Hosp, State Key Lab Med Genom,Shanghai Inst Hematol, Shanghai 200030, Peoples R China; 5.Ctr Dis Control & Prevent CDC, Biol Mass Spectrometry Lab, Atlanta, GA USA |
| 推荐引用方式 GB/T 7714 | Li, Keqin Kathy,Luo, Cheng,Wang, Dongxia,et al. Chemical and biochemical approaches in the study of histone methylation and demethylation[J]. MEDICINAL RESEARCH REVIEWS,2012,32(4):815-867. |
| APA | Li, Keqin Kathy,Luo, Cheng,Wang, Dongxia,Jiang, Hualiang,&Zheng, Y. George.(2012).Chemical and biochemical approaches in the study of histone methylation and demethylation.MEDICINAL RESEARCH REVIEWS,32(4),815-867. |
| MLA | Li, Keqin Kathy,et al."Chemical and biochemical approaches in the study of histone methylation and demethylation".MEDICINAL RESEARCH REVIEWS 32.4(2012):815-867. |
入库方式： OAI收割
来源：上海药物研究所
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