beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin from seeds of Cerbera manghas L. induces apoptosis in human hepatocellular carcinoma HepG2 cells
文献类型：期刊论文
| 作者 | Feng, Bo2; Guo, Yue-Wei1 ; Huang, Cai-Guo2; Li, Liang1; Jiao, Bing-Hua2
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| 刊名 | EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
 |
| 出版日期 | 2012-07 |
| 卷号 | 64期号:5页码:403-410 |
| 关键词 | beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin (GHSC-73) HepG2 Apoptosis-inducing factor (AIF) Mitochondrial membrane potential (Delta psi(m)) Caspase-3 Apoptosis |
| ISSN号 | 0940-2993 |
| DOI | 10.1016/j.etp.2010.10.005 |
| 文献子类 | Article |
| 英文摘要 | beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin (GHSC-73) is a cardiac glycoside isolated from the seeds of Cerbera manghas L. GHSC-73 reduced viability of HepG2 cells in a time- and dose-dependent manner without decreasing the viability of Chang human liver cells and Swiss albino 3T3 fibroblasts. induced efficiently stimulated apoptosis in HepG2 cells as evidenced by DNA fragmentation, annexin V/PI binding assay and DAPI staining. This apoptotic process was accompanied by the activation of the effector caspase-3, the loss of mitochondrial membrane potential (Delta psi(m)) and translocation of AIF from the mitochondrion to the nucleus in HepG2 cells. In addition, a broad-spectrum caspase inhibitor (z-VAD-fmk) tested in this experiment partially prevent HepG2 cells from GHSC-73-induced cell death, but did not affect translocation of AIF from the mitochondrion to the nucleus after GHSC-73 treatment. Our results firstly show that GHSC-73 inhibits the growth of HepG2 cells through caspase-dependent and -independent apoptosis pathways. (C) 2010 Elsevier GmbH. All rights reserved. |
| WOS关键词 | UNFOLDED PROTEIN RESPONSE ; CARDIAC-GLYCOSIDES ; DEATH ; MITOCHONDRIA ; MECHANISMS ; RECEPTOR ; DIGOXIN ; GROWTH |
| 资助项目 | National Basic Research Program of China (973 Program)[2010CB833804] |
| WOS研究方向 | Pathology ; Toxicology |
| 语种 | 英语 |
| WOS记录号 | WOS:000304513300001 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278036]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 上海药物代谢研究中心 |
| 通讯作者 | Huang, Cai-Guo |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China 2.Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Feng, Bo,Guo, Yue-Wei,Huang, Cai-Guo,et al. beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin from seeds of Cerbera manghas L. induces apoptosis in human hepatocellular carcinoma HepG2 cells[J]. EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY,2012,64(5):403-410. |
| APA | Feng, Bo,Guo, Yue-Wei,Huang, Cai-Guo,Li, Liang,&Jiao, Bing-Hua.(2012).beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin from seeds of Cerbera manghas L. induces apoptosis in human hepatocellular carcinoma HepG2 cells.EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY,64(5),403-410. |
| MLA | Feng, Bo,et al."beta-D-Glucosyl-(1-4)-alpha-L-thevetosides of 17 beta-digitoxigenin from seeds of Cerbera manghas L. induces apoptosis in human hepatocellular carcinoma HepG2 cells".EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY 64.5(2012):403-410. |
入库方式： OAI收割
来源：上海药物研究所
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