Different effects of sonoporation on cell morphology and viability
文献类型：期刊论文
| 作者 | Zhang, Ji-Zhen2; Saggar, Jasdeep K.1; Zhou, Zhao-Li3; Bing-Hu2 |
| 刊名 | BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES
 |
| 出版日期 | 2012-05 |
| 卷号 | 12期号:2页码:64-68 |
| 关键词 | sonoporation molecular delivery drug delivery ultrasound low frequency ultrasound microbubble contrast agents cell morphology |
| ISSN号 | 1512-8601 |
| 文献子类 | Article |
| 英文摘要 | The objective of our study was to investigate changes in cell morphology and viability after sonoporation. Sonoportion was achieved by ultrasound (21 kHz) exposure on adherent human prostate cancer DU145 cells in the cell culture dishes with the presence of microbubble contrast agents and calcein (a cell impermeant dye). We investigated changes in cell morphology immediately after sonoporation under scanning electron microscope (SEM) and changes in cell viability immediately and 6 h after sonoporation under fluorescence microscope. It was shown that various levels of intracellular calcein uptake and changes in cell morphology can be caused immediately after sonoporation: smooth cell surface, pores in the membrane and irregular cell surface. Immediately after sonoporation, both groups of cells with high levels of calcein uptake and low levels of calcein uptake were viable; 6 h after sonoporation, group of cells with low levels of calcein uptake still remained viable, while group of cells with high levels of calcein uptake died. Sonoporation induces different effects on cell morphology, intracellular calcein uptake and cell viability. (c) 2012 Association of Basic Medical Sciences of FBIH. All rights reserved |
| WOS关键词 | HAMSTER OVARY CELLS ; ACOUSTIC CAVITATION ; DRUG-DELIVERY ; INTRACELLULAR DELIVERY ; INERTIAL CAVITATION ; MEMBRANE POROSITY ; GENE DELIVERY ; ULTRASOUND ; MICROBUBBLES ; TRANSFECTION |
| 资助项目 | National Natural Science Foundation of China[30770562] ; Shanghai Science and the Technology Committee Basic Research Program[10JC1412600] |
| WOS研究方向 | Research & Experimental Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000305781100002 |
| 出版者 | ASSOC BASIC MEDICAL SCI FEDERATION BOSNIA & HERZEGOVINA SARAJEVO |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278084]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Bing-Hu |
| 作者单位 | 1.Univ Toronto, Dept Med Biophys, Toronto, ON M5S 3E2, Canada; 2.Shanghai Jiao Tong Univ, Dept Ultrasound Med, Affiliated Peoples Hosp 6, Shanghai Inst Ultrasound Med, Shanghai 200233, Peoples R China; 3.Chinese Acad Sci, Pharmaceut Holding Co Ltd, State Key Lab Drug Res, Cent Res Inst,Shanghai Inst Mat Med,Div Antitumor, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Ji-Zhen,Saggar, Jasdeep K.,Zhou, Zhao-Li,et al. Different effects of sonoporation on cell morphology and viability[J]. BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES,2012,12(2):64-68. |
| APA | Zhang, Ji-Zhen,Saggar, Jasdeep K.,Zhou, Zhao-Li,&Bing-Hu.(2012).Different effects of sonoporation on cell morphology and viability.BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES,12(2),64-68. |
| MLA | Zhang, Ji-Zhen,et al."Different effects of sonoporation on cell morphology and viability".BOSNIAN JOURNAL OF BASIC MEDICAL SCIENCES 12.2(2012):64-68. |
入库方式： OAI收割
来源：上海药物研究所
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