Dynamic monitoring of beta-cell injury with impedance and rescue by glucagon-like peptide-1
文献类型：期刊论文
| 作者 | Guan, Ni1; Gao, Weiwei1; He, Min1,2; Zheng, Min3; Xu, Xiao1,3; Wang, Xiaobo3; Wang, Ming-Wei1,2
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| 刊名 | ANALYTICAL BIOCHEMISTRY
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| 出版日期 | 2012-04-01 |
| 卷号 | 423期号:1页码:61-69 |
| 关键词 | xCELLigence system beta-Cell injury Cytokines Free fatty acids Streptozotocin Hydrogen peroxide Glucagon-like peptide-1 Boc5 |
| ISSN号 | 0003-2697 |
| DOI | 10.1016/j.ab.2012.01.014 |
| 文献子类 | Article |
| 英文摘要 | beta-Cell injury plays an important role in the development of type 1 and type 2 diabetes. Most of the beta-cell bioassays depend on labeling or endpoint assessments that might not capture the true physiology or pathology of the injury process. In the current study, we dynamically detected a broad range of pathological and pharmacological responses to four toxicants (cytokine mixture, free fatty acid mixture, streptozotocin, and hydrogen peroxide) in living beta-cells (INS-1E and MIN6) by a label-free, cell-based assay system named xCELLigence, codeveloped by ACEA Biosciences and Roche Diagnostics. Our results suggest that the impedance readout is highly sensitive and provides more information than some of the conventional endpoint cytotoxicity assays for beta-cell injury such as the Cell Counting Kit-8 (CCK-8) and morphology measurements. Furthermore, this system was used to evaluate the anti-injury effects of glucagon-like peptide-1 (GLP-1) and its nonpeptidic mimetic Boc5 by monitoring responses to four toxicants in two beta-cell lines. This study confirms that the protective property of Boc5 on beta-cells is similar to that of GLP-1. (C) 2012 Elsevier Inc. All rights reserved. |
| WOS关键词 | ACTIVATED PROTEIN-KINASE ; INSULIN-SECRETING CELLS ; INDUCED APOPTOSIS ; OXIDATIVE STRESS ; REAL-TIME ; GLUCOSE ; MICE ; RAT ; INHIBITION ; EXENDIN-4 |
| 资助项目 | Ministry of Science and Technology of China[2009ZX09302-001] ; Ministry of Science and Technology of China[2012ZX09304011] ; Shanghai Science and Technology Development Fund[11DZ2292200] ; CAS-Novo Nordisk[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000301759200010 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278140]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Wang, Ming-Wei |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.Hangzhou High Throughput Drug Screening Ctr, Hangzhou 310030, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guan, Ni,Gao, Weiwei,He, Min,et al. Dynamic monitoring of beta-cell injury with impedance and rescue by glucagon-like peptide-1[J]. ANALYTICAL BIOCHEMISTRY,2012,423(1):61-69. |
| APA | Guan, Ni.,Gao, Weiwei.,He, Min.,Zheng, Min.,Xu, Xiao.,...&Wang, Ming-Wei.(2012).Dynamic monitoring of beta-cell injury with impedance and rescue by glucagon-like peptide-1.ANALYTICAL BIOCHEMISTRY,423(1),61-69. |
| MLA | Guan, Ni,et al."Dynamic monitoring of beta-cell injury with impedance and rescue by glucagon-like peptide-1".ANALYTICAL BIOCHEMISTRY 423.1(2012):61-69. |
入库方式： OAI收割
来源：上海药物研究所
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