Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line
文献类型：期刊论文
| 作者 | Lu, Jin-Jian1,2; Chen, Si-Meng1; Ding, Jian1 ; Meng, Ling-Hua1
|
| 刊名 | MOLECULAR AND CELLULAR BIOCHEMISTRY
 |
| 出版日期 | 2012-01 |
| 卷号 | 360期号:1-2页码:329-337 |
| 关键词 | Dihydroartemisinin Resistant Proteomics Anti-tumor HCT116 |
| ISSN号 | 0300-8177 |
| DOI | 10.1007/s11010-011-1072-2 |
| 文献子类 | Article |
| 英文摘要 | Dihydroartemisinin (DHA) is an important artemisinin derivative and presents profound anti-tumor potential. A DHA-resistant cell line named HCT116/R derived from colon carcinoma cell line HCT116 was established in our previous study. Herein, we found that HCT116/R cells were much more resistant to DHA- or artesunate-induced proliferation inhibition and more tolerant to DHA-induced cell cycle arrest and apoptosis compared with those of the parent HCT116 cells. The protein levels of P-glycoprotein and MDR-associated protein 1 and the accumulation of doxorubicin in cells were similar in both cell lines. Moreover, HCT116/R cells were still sensitive to camptothecin- and doxorubicin-induced cell growth inhibition. To further explore the characterization of HCT116/R cell line, a proteomic study employing two-dimensional gel electrophoresis and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry was performed. Eight different expressed proteins between the two cell lines were identified including some heat shock proteins, annexins, etc. This study not only indicates that exposure to DHA may not induce a tumor multi-drug-resistant phenotype but also affords new clues for the further investigation of the anti-cancer mechanisms of DHA and other artemisinin derivatives. |
| WOS关键词 | PANCREATIC-CANCER CELLS ; OVARIAN-CANCER ; IN-VITRO ; BORTEZOMIB RESISTANCE ; MULTIDRUG-RESISTANCE ; PLATINUM-RESISTANCE ; PROTEOMIC ANALYSIS ; LEUKEMIA-CELLS ; ARTEMISININ ; APOPTOSIS |
| 资助项目 | National Natural Science of China[81001450] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000297722800035 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278296]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Ding, Jian |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Zhejiang Chinese Med Univ, Coll Life Sci, Hangzhou 310053, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lu, Jin-Jian,Chen, Si-Meng,Ding, Jian,et al. Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line[J]. MOLECULAR AND CELLULAR BIOCHEMISTRY,2012,360(1-2):329-337. |
| APA | Lu, Jin-Jian,Chen, Si-Meng,Ding, Jian,&Meng, Ling-Hua.(2012).Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line.MOLECULAR AND CELLULAR BIOCHEMISTRY,360(1-2),329-337. |
| MLA | Lu, Jin-Jian,et al."Characterization of dihydroartemisinin-resistant colon carcinoma HCT116/R cell line".MOLECULAR AND CELLULAR BIOCHEMISTRY 360.1-2(2012):329-337. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。