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Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells
文献类型：期刊论文
| 作者 | Wang, Quan1; Xu, Xinxiu2; Li, Jun3 ; Liu, Jing4; Gu, Haifeng1; Zhang, Ru2; Chen, Jiekai4; Kuang, Yin5; Fei, Jian5; Jiang, Cong6,7
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| 刊名 | CELL RESEARCH
 |
| 出版日期 | 2011-10 |
| 卷号 | 21期号:10页码:1424-1435 |
| 关键词 | lithium induced pluripotent stem cells iPS GSK3 beta Nanog LSD1 histone demethylase |
| ISSN号 | 1001-0602 |
| DOI | 10.1038/cr.2011.108 |
| 文献子类 | Article |
| 英文摘要 | Somatic cells can be reprogrammed into induced pluripotent stem cells (iPSCs) by defined factors. The low efficiency of reprogramming and genomic integration of oncogenes and viral vectors limited the potential application of iPSCs. Here we report that Lithium (Li), a drug used to treat mood disorders, greatly enhances iPSC generation from both mouse embryonic fibroblast and human umbilical vein endothelial cells. Li facilitates iPSC generation with one (Oct4) or two factors (OS or OK). The effect of Li on promoting reprogramming only partially depends on its major target GSK3 beta. Unlike other GSK3 beta inhibitors, Li not only increases the expression of Nanog, but also enhances the transcriptional activity of Nanog. We also found that Li exerts its effect by promoting epigenetic modifications via downregulation of LSD1, a H3K4-specific histone demethylase. Knocking down LSD1 partially mimics Li's effect in enhancing reprogramming. Our results not only provide a straightforward method to improve the iPSC generation efficiency, but also identified a histone demethylase as a critical modulator for somatic cell reprogramming. |
| WOS关键词 | MOUSE FIBROBLASTS ; EXPRESSION ; NANOG ; OCT4 ; P53 |
| 资助项目 | Chinese Academy of Sciences[XDA01040301] ; Ministry of Science and Technology of China[2009CB940900] ; Ministry of Science and Technology of China[2009ZX09302-001] ; National Natural Science Foundation of China[90713047] ; Shanghai Commission of Science and Technology[08DZ2291300] ; Shanghai Commission of Science and Technology[09DZ2291200] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| CSCD记录号 | CSCD:4321609 |
| WOS记录号 | WOS:000295457400007 |
| 出版者 | INST BIOCHEMISTRY & CELL BIOLOGY |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278388]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Xie, Xin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Tongji Univ, Sch Life Sci & Technol, Lab Receptor Based Biomed, Shanghai Key Lab Signaling & Dis Res, Shanghai 200092, Peoples R China; 3.Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA; 4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, S China Inst Stem Cell Biol & Regenerat Med, Key Lab Regenerat Biol, Guangzhou, Guangdong, Peoples R China; 5.Shanghai Res Ctr Biomodel Organism, Shanghai, Peoples R China; 6.E China Normal Univ, Inst Biomed Sci, Shanghai 200062, Peoples R China; 7.E China Normal Univ, Sch Life Sci, Shanghai 200062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Quan,Xu, Xinxiu,Li, Jun,et al. Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells[J]. CELL RESEARCH,2011,21(10):1424-1435. |
| APA | Wang, Quan.,Xu, Xinxiu.,Li, Jun.,Liu, Jing.,Gu, Haifeng.,...&Xie, Xin.(2011).Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells.CELL RESEARCH,21(10),1424-1435. |
| MLA | Wang, Quan,et al."Lithium, an anti-psychotic drug, greatly enhances the generation of induced pluripotent stem cells".CELL RESEARCH 21.10(2011):1424-1435. |
入库方式： OAI收割
来源：上海药物研究所
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