Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases
文献类型：期刊论文
| 作者 | Liu, Tian2; Zhang, Haitao1; Liu, Fengyi2; Chen, Lei2; Shen, Xu1 ; Yang, Qing2
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| 刊名 | BIOCHEMICAL JOURNAL
 |
| 出版日期 | 2011-09-15 |
| 卷号 | 438页码:467-474 |
| 关键词 | beta-N-acetyl-D-hexosaminidase bactericide inhibitor insect pesticide O-(2-acetamido-2-deoxy-D-glucopyransylidene)-amino-N-phenylcarbamate (PUGNAc) |
| ISSN号 | 0264-6021 |
| DOI | 10.1042/BJ20110390 |
| 文献子类 | Article |
| 英文摘要 | Chitinolytic beta-N-acetyl-D-hexosaminidase is a branch of the GH20 (glycoside hydrolase family 20) beta-N-acetyl-D-hexosaminidases that is only distributed in insects and micro-organisms, and is therefore a potential target for the action of insecticides. PUGNAc [O-(2-acetamido-2-deoxy-D-glucopyransylidene)-amino-N-phenylcarbamate] was initially identified as an inhibitor against GH20 beta-N-acetyl-D-hexosaminidases. So far no crystal structure of PUGNAc in complex with any GH20 beta-N-acetyl-D-hexosaminidase has been reported. We show in the present study that the sensitivities of chitinolytic fi-N-acetyl-D-hexosaminidases towards PUGNAc can vary by 100-fold, with the order being OfHex 1 (Ostrinia furnacalis beta-N-acetyl-D-hexosaminidase) |
| WOS关键词 | SUBSTRATE-ASSISTED CATALYSIS ; TAY-SACHS-DISEASE ; OSTRINIA-FURNACALIS ; TMG-CHITOTRIOMYCIN ; HYDROLYSIS ; ACETYLGLUCOSAMINIDASES ; PURIFICATION ; INHIBITION ; CHITOBIASE ; MECHANISM |
| 资助项目 | National Key Project for Basic Research[2010CB126100] ; National Natural Science Foundation of China[31070715] ; National High Technology Research and Development Program of China[2011AA10A204] ; National Key Technology RD Program[2011BAE06B05] ; Fundamental Research Funds for the Central Universities[DUT11ZD113] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000295182100008 |
| 出版者 | PORTLAND PRESS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278401]  |
| 专题 | 药理学第三研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Shen, Xu |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Tian,Zhang, Haitao,Liu, Fengyi,et al. Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases[J]. BIOCHEMICAL JOURNAL,2011,438:467-474. |
| APA | Liu, Tian,Zhang, Haitao,Liu, Fengyi,Chen, Lei,Shen, Xu,&Yang, Qing.(2011).Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases.BIOCHEMICAL JOURNAL,438,467-474. |
| MLA | Liu, Tian,et al."Active-pocket size differentiating insectile from bacterial chitinolytic beta-N-acetyl-D-hexosaminidases".BIOCHEMICAL JOURNAL 438(2011):467-474. |
入库方式： OAI收割
来源：上海药物研究所
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