TRAPPC4-ERK2 Interaction Activates ERK1/2, Modulates Its Nuclear Localization and Regulates Proliferation and Apoptosis of Colorectal Cancer Cells
文献类型：期刊论文
| 作者 | Zhao, Shu-Liang1; Hong, Jie1; Xie, Zuo-Quan2 ; Tang, Jie-Ting1; Su, Wen-Yu1; Du, Wan1; Chen, Ying-Xuan1; Lu, Rong1; Sun, Dan-Feng1; Fang, Jing-Yuan1
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| 刊名 | PLOS ONE
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| 出版日期 | 2011-08-03 |
| 卷号 | 6期号:8 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0023262 |
| 文献子类 | Article |
| 英文摘要 | The trafficking protein particle complex 4 (TRAPPC4) is implicated in vesicle-mediated transport, but its association with disease has rarely been reported. We explored its potential interaction with ERK2, part of the ERK1/2 complex in the Extracellular Signal-regulated Kinase/Mitogen-activated Protein Kinase (ERK-MAPK) pathway, by a yeast two-hybrid screen and confirmed by co-immunoprecipitation (Co-IP) and glutathione S-transferase (GST) pull-down. Further investigation found that when TRAPPC4 was depleted, activated ERK1/2 specifically decreased in the nucleus, which was accompanied with cell growth suppression and apoptosis in colorectal cancer (CRC) cells. Overexpression of TRAPPC4 promoted cell viability and caused activated ERK1/2 to increase overall, but especially in the nucleus. TRAPPC4 was expressed more highly in the nucleus of CRC cells than in normal colonic epithelium or adenoma which corresponded with nuclear staining of pERK1/2. We demonstrate here that TRAPPC4 may regulate cell proliferation and apoptosis in CRC by interaction with ERK2 and subsequently phosphorylating ERK1/2 as well as modulating the subcellular location of pERK1/2 to activate the relevant signaling pathway. |
| WOS关键词 | SPONDYLOEPIPHYSEAL DYSPLASIA TARDA ; TRAPP COMPLEX ; MAP KINASE ; PROTEIN ; GOLGI ; SYNDECANS ; TRANSPORT ; GENE ; PHOSPHORYLATION ; ARCHITECTURE |
| 资助项目 | National Natural Science Foundation of Key Program[30830055] ; National Natural Science Foundation of Youth Program[31000634] ; Shanghai Commission for Science and Technology[10ZR1419000] ; Shanghai Health Bureau for youth[2009032] ; Shanghai Jiao-Tong University School of Medicine Foundation for Science and Technology[09XJ21065] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000293558900070 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278448]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhao, Shu-Liang |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Ren Ji Hosp, Shanghai Inst Digest Dis,Dept Gastroenterol, Shanghai 200030, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Shu-Liang,Hong, Jie,Xie, Zuo-Quan,et al. TRAPPC4-ERK2 Interaction Activates ERK1/2, Modulates Its Nuclear Localization and Regulates Proliferation and Apoptosis of Colorectal Cancer Cells[J]. PLOS ONE,2011,6(8). |
| APA | Zhao, Shu-Liang.,Hong, Jie.,Xie, Zuo-Quan.,Tang, Jie-Ting.,Su, Wen-Yu.,...&Fang, Jing-Yuan.(2011).TRAPPC4-ERK2 Interaction Activates ERK1/2, Modulates Its Nuclear Localization and Regulates Proliferation and Apoptosis of Colorectal Cancer Cells.PLOS ONE,6(8). |
| MLA | Zhao, Shu-Liang,et al."TRAPPC4-ERK2 Interaction Activates ERK1/2, Modulates Its Nuclear Localization and Regulates Proliferation and Apoptosis of Colorectal Cancer Cells".PLOS ONE 6.8(2011). |
入库方式： OAI收割
来源：上海药物研究所
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