Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-alpha and IL-6 in LPS-Induced Macrophages
文献类型：期刊论文
| 作者 | Tang, Jin-Shan1; Zhao, Feng2; Gao, Hao1,3; Dai, Yi1; Yao, Zhi-Hong1; Hong, Kui4; Li, Jia3,5 ; Ye, Wen-Cai1; Yao, Xin-Sheng1
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| 刊名 | MARINE DRUGS
 |
| 出版日期 | 2010-10 |
| 卷号 | 8期号:10页码:2605-2618 |
| 关键词 | LC-MS Bacillus sp. surfactin isomer anti-inflammatory activity |
| ISSN号 | 1660-3397 |
| DOI | 10.3390/md8102605 |
| 文献子类 | Article |
| 英文摘要 | A rapid method for characterization and online detection of surfactin isomers was developed based on HPLC-MSn (n = 1, 2, 3) analyses, and many surfactin isomers were detected and characterized from the bioactive fraction of the mangrove bacterium Bacillus sp. Inhibitory activities of surfactin isomers on the overproduction of nitric oxide and the release of TNF-alpha and IL-6 in LPS-induced macrophages were systematically investigated. It was revealed that the surfactin isomers showed strong inhibitory properties on the overproduction of nitric oxide and the release of IL-6 on LPS-induced murine macrophage cell RAW264.7 with IC50 values ranging from 1.0 to 7.0. mu M. Structure-activity relationship (SAR) studies revealed that the existence of the free carboxyl group in the structure of surfactin isomers was crucial. These findings will be very helpful for the development of this novel kind of natural product as new anti-inflammatory agents. |
| WOS关键词 | TANDEM MASS-SPECTROMETRY ; CYCLIC LIPOPEPTIDE ; BACILLUS-SUBTILIS ; MECHANISM ; CELLS |
| 资助项目 | National Natural Science Foundation of China[U0633008] ; Ministry of Science and Technology of China[2009CB522300] ; Ministry of Science and Technology of China[2008ZX09401-05] ; Ministry of Science and Technology of China[2009ZX09302-004] ; Ministry of Education of China/Fok Ying Tung Education Foundation[121039] ; China Postdoctoral Funds[20090450886] ; State Key Laboratory of Drug Research[SIMM0812KF-02] |
| WOS研究方向 | Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000285711800005 |
| 出版者 | MDPI AG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278763]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药物安全性评价中心 |
| 通讯作者 | Yao, Xin-Sheng |
| 作者单位 | 1.Jinan Univ, Inst Tradit Chinese Med & Nat Prod, Guangzhou 510632, Guangdong, Peoples R China; 2.Yantai Univ, Sch Pharm, Yantai 206005, Shandong, Peoples R China; 3.Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China; 5.Shanghai Inst Mat Med, Natl Ctr Drug Screening, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tang, Jin-Shan,Zhao, Feng,Gao, Hao,et al. Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-alpha and IL-6 in LPS-Induced Macrophages[J]. MARINE DRUGS,2010,8(10):2605-2618. |
| APA | Tang, Jin-Shan.,Zhao, Feng.,Gao, Hao.,Dai, Yi.,Yao, Zhi-Hong.,...&Yao, Xin-Sheng.(2010).Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-alpha and IL-6 in LPS-Induced Macrophages.MARINE DRUGS,8(10),2605-2618. |
| MLA | Tang, Jin-Shan,et al."Characterization and Online Detection of Surfactin Isomers Based on HPLC-MSn Analyses and Their Inhibitory Effects on the Overproduction of Nitric Oxide and the Release of TNF-alpha and IL-6 in LPS-Induced Macrophages".MARINE DRUGS 8.10(2010):2605-2618. |
入库方式： OAI收割
来源：上海药物研究所
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