Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy
文献类型：期刊论文
| 作者 | Li Mi1,5,6; Liu LianQing1; Xi Ning1,2; Wang YueChao1; Dong ZaiLi1; Li GuangYong1,3; Xiao XiuBin4; Zhang WeiJing4 |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2010-10 |
| 卷号 | 53期号:10页码:1189-1195 |
| 关键词 | atomic force microscopy single-molecule force spectroscopy CD20 antigen Rituximab |
| ISSN号 | 1674-7305 |
| DOI | 10.1007/s11427-010-4070-9 |
| 文献子类 | Article |
| 英文摘要 | Elucidating the underlying mechanisms of cell physiology is currently an important research topic in life sciences. Atomic force microscopy methods can be used to investigate these molecular mechanisms. In this study, single-molecule force spectroscopy was used to explore the specific recognition between the CD20 antigen and anti-CD20 antibody Rituximab on B lymphoma cells under near-physiological conditions. The CD20-Rituximab specific binding force was measured through tip functionalization. Distribution of CD20 on the B lymphoma cells was visualized three-dimensionally. In addition, the relationship between the intramolecular force and the molecular extension of the CD20-Rituximab complex was analyzed under an external force. These results facilitate further investigation of the mechanism of Rituximab's anti-cancer effect. |
| WOS关键词 | MONOCLONAL-ANTIBODY THERAPY ; SINGLE-MOLECULE ; LIVING CELLS ; RECOGNITION EVENTS ; CANCER ; RESOLUTION ; RECEPTOR ; SURFACE ; LOCALIZATION ; SPECTROSCOPY |
| 资助项目 | National Natural Science Foundation of China[60904095] ; National Natural Science Foundation of China[60635040] ; National High Technology Research and Development Program of China[2009AA03Z316] ; CAS FEA[00000000] ; State Key Laboratory of Drug Research[00000000] |
| WOS研究方向 | Life Sciences & Biomedicine - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000283120000005 |
| 出版者 | SCIENCE PRESS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278770]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Liu LianQing |
| 作者单位 | 1.Chinese Acad Sci, Shenyang Inst Automat, State Key Lab Robot, Shenyang 110016, Peoples R China; 2.Michigan State Univ, Dept Elect & Comp Engn, E Lansing, MI 48824 USA; 3.Univ Pittsburgh, Dept Elect & Comp Engn, Pittsburgh, PA 15261 USA; 4.Mil Med Acad Sci, Affiliated Hosp, Dept Lymphoma, Beijing 100071, Peoples R China; 5.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China; 6.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Mi,Liu LianQing,Xi Ning,et al. Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy[J]. SCIENCE CHINA-LIFE SCIENCES,2010,53(10):1189-1195. |
| APA | Li Mi.,Liu LianQing.,Xi Ning.,Wang YueChao.,Dong ZaiLi.,...&Zhang WeiJing.(2010).Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy.SCIENCE CHINA-LIFE SCIENCES,53(10),1189-1195. |
| MLA | Li Mi,et al."Detecting CD20-Rituximab specific interactions on lymphoma cells using atomic force microscopy".SCIENCE CHINA-LIFE SCIENCES 53.10(2010):1189-1195. |
入库方式： OAI收割
来源：上海药物研究所
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