Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase C alpha
文献类型：期刊论文
| 作者 | Peng, HongLi1 ; Yaney, Gordon C.3,4; Kirber, Michael T.2,4
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| 刊名 | PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
 |
| 出版日期 | 2010-09 |
| 卷号 | 460期号:4页码:791-802 |
| 关键词 | Protein kinase C Ryanodine receptor Calcium Caffeine |
| ISSN号 | 0031-6768 |
| DOI | 10.1007/s00424-010-0850-0 |
| 文献子类 | Article |
| 英文摘要 | The role of protein kinase C (PKC) in Ca2+ release through ryanodine receptors (RyRs) in the sarcoplasmic reticulum (SR) of vascular smooth muscle cells (SMCs) is not well understood. Caffeine was used to activate RyRs and the intracellular Ca2+ concentration ([Ca2+](i)) was measured in both freshly isolated and cultured mouse aortic SMCs (ASMCs). Pre-activation of PKC with 1,2-dioctanoyl-sn-glycerol (DOG) prevented caffeine-induced [Ca2+](i) transients. Application of the PKC inhibitor calphostin C caused [Ca2+](i) transients which were not blocked by nifedipine or by removing extracellular Ca2+ but were abolished after inhibition of the SR Ca2+-ATPase with thapsigargin or after inhibition of RyRs with ryanodine. In addition, chelerythrine and GF109203X also elevated resting [Ca2+](i) but no further [Ca2+](i) increase was seen with subsequent application of caffeine. Selective inhibition of PKC alpha with safingol blocked caffeine-induced [Ca2+](i) transients, but the PKC epsilon inhibitory peptide V1-2 did not. In cells expressing a EGFP-tagged PKC alpha, caffeine-induced [Ca2+](i) transients were associated with a rapid focal translocation near the cell periphery, while application of ionomycin and DOG caused translocation to the plasma membrane. Western blot showed that caffeine increased the relative amount of PKC alpha in the particulate fraction in a time-dependent manner. Co-immunoprecipitation of RyRs and PKC alpha indicated that they interact. In conclusion, our studies suggest that PKC activation can inhibit the gating activity of RyRs in the SR of ASMCs, and this regulation is most likely mediated by the Ca2+-dependent PKC alpha isoform. |
| WOS关键词 | SKELETAL-MUSCLE ; INTRACELLULAR CALCIUM ; SARCOPLASMIC-RETICULUM ; SIGNAL-TRANSDUCTION ; INDUCED CONTRACTION ; HUMAN-NEUTROPHILS ; IN-SITU ; CELLS ; PHOSPHORYLATION ; ACTIVATION |
| 资助项目 | National Natural Science Foundation of China[30672467] ; Ministry of Science and Technology[2007AA02Z308] ; American Diabetes Association[7-05-JF-53] ; NIH[DK35914] ; NIH[DK47223] ; Evans Department of Medicine, Boston University School of Medicine[00000000] |
| WOS研究方向 | Physiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000280920900010 |
| 出版者 | SPRINGER |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278792]  |
| 专题 | 药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Kirber, Michael T. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Boston Univ, Med Ctr, Whitaker Cardiovasc Inst, Boston, MA 02118 USA; 3.Boston Univ, Med Ctr, Obes Res Ctr, Boston, MA 02118 USA; 4.Boston Univ, Med Ctr, Dept Med, Sect Mol Med, Boston, MA 02118 USA |
| 推荐引用方式 GB/T 7714 | Peng, HongLi,Yaney, Gordon C.,Kirber, Michael T.. Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase C alpha[J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY,2010,460(4):791-802. |
| APA | Peng, HongLi,Yaney, Gordon C.,&Kirber, Michael T..(2010).Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase C alpha.PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY,460(4),791-802. |
| MLA | Peng, HongLi,et al."Modulation of Ca2+ release through ryanodine receptors in vascular smooth muscle by protein kinase C alpha".PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY 460.4(2010):791-802. |
入库方式： OAI收割
来源：上海药物研究所
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