Determination of dehydroandrographolide succinate in human plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS): Method development, validation and clinical pharmacokinetic study
文献类型：期刊论文
| 作者 | Li, Shuijun1,2; Yang, Ding2; Zhang, Mengqi1; Zhou, Juzhen1; Li, Rui3; Lu, Chuan1; Liu, Yun1; Liu, Yanmei1; Wang, Yiping2 ; Yu, Chen1
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2010-08-15 |
| 卷号 | 878期号:24页码:2274-2279 |
| 关键词 | Dehydroandrographolide succinate Protein precipitation LC-MS/MS Pharmacokinetic study Human plasma |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2010.06.030 |
| 文献子类 | Article |
| 英文摘要 | A LC-MS/MS method was developed and validated for the determination of dehydroandrographolide succinate (DAS), a traditional Chinese medicine derivative used for the treatment of pneumonia, upper respiratory tract infection, and chronic bronchitis. Following protein precipitation, DAS was detected by ion transition at m/z 531.2/99.0 in multiple reaction monitoring mode with negative electrospray ionization-tandem mass spectrometry. The limit of detection was 0.5 ng/mL, and the lower limit of quantification was 1 0 ng/mL in human plasma. Good linearity was maintained over the range of 10-5000 ng/mL, and the correlation coefficient was better than 0.99. The accuracy ranged from 95.3% to 113%, RSD from 0.928% to 6.47%, for the within- and between-run analysis at all QC levels. The recovery ranged from 85.5% to 93.4% and the matrix effect from 107% to 119%. No significant carryover and good stability were found during method validation. The developed method was successfully applied to the determination of DAS in human plasma in a pharmacokinetic study following intravenous infusion of potassium sodium DAS to nine healthy volunteers. (C) 2010 Elsevier B.V. All rights reserved. |
| 资助项目 | National Science & Technology Major Project, China[2009ZX09301-001] ; National Science & Technology Major Project, China[08411966700] ; Science and Technology Commission of Shanghai Municipality, Shanghai, China[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000281290400014 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278798]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yu, Chen |
| 作者单位 | 1.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 3.YaoPharma Co Ltd, Chongqing 401121, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Shuijun,Yang, Ding,Zhang, Mengqi,et al. Determination of dehydroandrographolide succinate in human plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS): Method development, validation and clinical pharmacokinetic study[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(24):2274-2279. |
| APA | Li, Shuijun.,Yang, Ding.,Zhang, Mengqi.,Zhou, Juzhen.,Li, Rui.,...&Yu, Chen.(2010).Determination of dehydroandrographolide succinate in human plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS): Method development, validation and clinical pharmacokinetic study.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(24),2274-2279. |
| MLA | Li, Shuijun,et al."Determination of dehydroandrographolide succinate in human plasma by liquid chromatography tandem mass spectrometry (LC-MS/MS): Method development, validation and clinical pharmacokinetic study".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.24(2010):2274-2279. |
入库方式： OAI收割
来源：上海药物研究所
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