Activation of phosphatidylinositol-linked D1-like receptors increases spontaneous glutamate release in rat somatosensory cortical neurons in vitro
文献类型：期刊论文
| 作者 | Chu, Hong-Yuan1,2; Yang, Zhi; Zhao, Bin; Jin, Guo-Zhang1,2 ; Hu, Guo-Yuan1,2; Zhen, Xuechu1,2
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| 刊名 | BRAIN RESEARCH
 |
| 出版日期 | 2010-07-09 |
| 卷号 | 1343页码:20-27 |
| ISSN号 | 0006-8993 |
| 关键词 | Dopamine receptor Glutamate release Protein kinase C SKF83959 Spontaneous excitatory postsynaptic current Transient receptor potential vanilloid 1 |
| DOI | 10.1016/j.brainres.2010.04.043 |
| 文献子类 | Article |
| 英文摘要 | Central dopaminergic system exerts profound modulation on spontaneous glutamate release in various brain regions mainly through D-1 receptor/cAMP/PKA pathway. It remains unclear whether the phosphatidylinositol (PI)-linked D-1-like receptors are also involved in such modulatory actions. The identification of substituted phenylbenzazepine SKF83959 as the selective agonist for the atypical D-1-like receptors has given impetus to study their influence on the spontaneous glutamate release in the brain. In the present study the effects of SKF83959 on the spontaneous excitatory postsynaptic currents (sEPSCs) were investigated through whole-cell recording from layer V-VI pyramidal neurons in rat somatosensory cortical slices. Perfusion with SKF83959 (10-100 mu M) considerably increased the frequency of sEPSCs, while had no significant effect on the amplitude of sEPSCs. The increase of sEPSC frequency by SKF83959 was blocked by SCH23390, a D-1-like receptor antagonist, but not by the antagonists for D-2 receptor, alpha(1)-adrenoceptor and 5-HT2A/2C receptor. U-73122 (PLC beta inhibitor), 2-APB (IP3 receptor antagonist), chelerythrine chloride (PKC inhibitor) and capsazepine (TRPV1 antagonist) could block the effects of SKF83959, whereas H-89 (PKA inhibitor) and forskolin (adenylyl cyclase activator) had no effect. Taken together, sensitization of TRPV1 channels by PKC after activation of D-1 receptor/PLC beta signaling pathway mediated SKF83959-induced increase in the sEPSC frequency. To our knowledge, this is the first pharmacological evidence that PI-linked D-1-like dopamine receptors do exist in presynaptic terminals of cortical neurons and play an important role in controlling the spontaneous glutamate release. (C) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | EXCITATORY SYNAPTIC-TRANSMISSION ; D1 DOPAMINE-RECEPTOR ; ADENYLATE-CYCLASE ; CORTEX ; SKF83959 ; INHIBITION ; STRIATUM ; BRAIN ; SCHIZOPHRENIA ; STIMULATION |
| 资助项目 | Ministry of Science and Technology of China[2007AA02z163] ; Ministry of Science and Technology of China[2009CB522001] ; Natural Science Foundation of China[20872153] ; Natural Science Foundation of China[30825042] ; Natural Science Foundation of China[30770662] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| 出版者 | ELSEVIER SCIENCE BV |
| WOS记录号 | WOS:000279806000003 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278835]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhen, Xuechu |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Chinese Acad Sci, Dept Pharmacol 2, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chu, Hong-Yuan,Yang, Zhi,Zhao, Bin,et al. Activation of phosphatidylinositol-linked D1-like receptors increases spontaneous glutamate release in rat somatosensory cortical neurons in vitro[J]. BRAIN RESEARCH,2010,1343:20-27. |
| APA | Chu, Hong-Yuan,Yang, Zhi,Zhao, Bin,Jin, Guo-Zhang,Hu, Guo-Yuan,&Zhen, Xuechu.(2010).Activation of phosphatidylinositol-linked D1-like receptors increases spontaneous glutamate release in rat somatosensory cortical neurons in vitro.BRAIN RESEARCH,1343,20-27. |
| MLA | Chu, Hong-Yuan,et al."Activation of phosphatidylinositol-linked D1-like receptors increases spontaneous glutamate release in rat somatosensory cortical neurons in vitro".BRAIN RESEARCH 1343(2010):20-27. |
入库方式： OAI收割
来源：上海药物研究所
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