Oligomannurarate sulfate, a novel antimitotic agent, exerts anti-cancer activity by binding to tubulin on novel site
文献类型：期刊论文
| 作者 | Miao, Benchun1; Chen, Yi2 ; Li, Ling1; Xin, Xianliang1; Gan, Li1; Fu, Xueyan1; Geng, Meiyu1,2
|
| 刊名 | CANCER BIOLOGY & THERAPY
 |
| 出版日期 | 2010-07-01 |
| 卷号 | 10期号:1页码:89-98 |
| 关键词 | JG3 tubulin antimicrotubule agent G(2)/M antineoplastic agent |
| ISSN号 | 1538-4047 |
| DOI | 10.4161/cbt.10.1.12167 |
| 文献子类 | Article |
| 英文摘要 | Tubulin-binding agents have received considerable interest as potential anti-cancer drugs. These compounds interfere with tubulin dynamics and microtubule organization and thereby induce cell growth arrest at G(2)/M phase, and eventually lead to apoptotic cell death. Herein, we report that sulfated oligosaccharide JG3 was effective at inhibiting viability of cancer cells, and suppressing tumor growth in vivo. Our studies showed that JG3 significantly arrested cell cycle at G(2)/M phase and led cancer cells to apoptotic death. Consistent with this, tubulin was identified as a binding protein of JG3 using affinity chromatography and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The interaction between JG3 and tubulin was characterized by surface plasmon resonance (SPR) analysis. Furthermore, it was demonstrated both at cellular level and in cell free system that JG3 potently prevented tubulin polymerization, thereby demolished microtubule organization in cancer cells. The underlying mechanism lies in its binding to tubulin on a unique site distinct from other conventional binding sites. All these suggest that JG3 is a novel antimitotic reagent, and this might shed new light on the understanding of anti-cancer activities and mechanisms of novel antimitotic reagents, and help develop new antimitotic agents in cancer therapy. |
| WOS关键词 | ACTIVITY IN-VIVO ; MICROTUBULE DYNAMICS ; TARGET ; CELLS ; RESISTANCE ; SPINDLE ; PROTEIN ; DRUGS ; MECHANISMS ; PACLITAXEL |
| 资助项目 | Natural Science Foundation of China for Distinguished Young Scholars[30725046] ; National Basic Research Program Grant of China[2003CB716400] |
| WOS研究方向 | Oncology |
| 语种 | 英语 |
| WOS记录号 | WOS:000279593900014 |
| 出版者 | TAYLOR & FRANCIS INC |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278849]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Geng, Meiyu |
| 作者单位 | 1.Ocean Univ China, Marine Drug & Food Inst, Dept Pharmacol, Qingdao, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Div Antitumor Pharmacol, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Miao, Benchun,Chen, Yi,Li, Ling,et al. Oligomannurarate sulfate, a novel antimitotic agent, exerts anti-cancer activity by binding to tubulin on novel site[J]. CANCER BIOLOGY & THERAPY,2010,10(1):89-98. |
| APA | Miao, Benchun.,Chen, Yi.,Li, Ling.,Xin, Xianliang.,Gan, Li.,...&Geng, Meiyu.(2010).Oligomannurarate sulfate, a novel antimitotic agent, exerts anti-cancer activity by binding to tubulin on novel site.CANCER BIOLOGY & THERAPY,10(1),89-98. |
| MLA | Miao, Benchun,et al."Oligomannurarate sulfate, a novel antimitotic agent, exerts anti-cancer activity by binding to tubulin on novel site".CANCER BIOLOGY & THERAPY 10.1(2010):89-98. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。