RP -HPLC determination of dobesilic acid in human plasma
文献类型：期刊论文
| 作者 | ZHANG Yini1; CHEN Xiaoyan1 ; YANG Hongmei2; ZHONG Dafang1
|
| 刊名 | Chinese Journal of Pharmaceutical Analysis
 |
| 出版日期 | 2007 |
| 卷号 | 27期号:8页码:1208-1211 |
| 关键词 | calcium dobesilate HPLC plasma drug concentration |
| ISSN号 | 0254-1793 |
| 其他题名 | 反相离子对高效液相色谱法测定人血浆中羟苯磺酸 |
| 文献子类 | Article |
| 英文摘要 | Objective：To develop and validate a sensitive and simple reversed -phase ion -pair HPLC method for the determination of dobesilic acid in human plasma. Methods：Mter a simple protein precipitation with acetonitrile, the analyte and the internal standard ,p -aminobenzoic acid ,were chromatographied on a Diamonsil C_(18) column with the mobile phase consisting of 0. 05 mol ·L~(-1), potassium dihydrogen phosphate buffer （ pH = 3.0） - acetonitrile （87： 13, v/v） , containing 0. 1% tetra - n - butylammonium hydroxide, at a flow rate of 1.0 mL ·min~( - 1). The UV wavelength was set at 300 nm. Results：The linear calibration curves were obtained in the range of 0. 364 -36.4 mg · L~(-1). The lower limit of quantification was 0. 364 mg·L~(-1). The inter -and intra -day RSD acrossed the three validation runs were both below 8.7% ,and the accuracy （RE） was within 4. 5% for the analyte. The mean recoveries of dobesilic acid and internal standard p- aminobenzoic acid were 74.8% and 94. 8% ,respectively. Conclusion：The method is proved to be special, convenient, and suitable for clinical investigation and pharmacokinetics of calcium dobesilate. |
| WOS研究方向 | Pharmacology & Pharmacy (provided by Clarivate Analytics) |
| 语种 | 中文 |
| CSCD记录号 | CSCD:2954751 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/268170]  |
| 专题 | 上海药物代谢研究中心 药物发现与设计中心 药物化学研究室 药理学第一研究室 |
| 通讯作者 | ZHONG Dafang |
| 作者单位 | 1.Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China.; 2.Shenyang Pharmaceutical University, Shenyang, Liaoning 110016, China. |
| 推荐引用方式 GB/T 7714 | ZHANG Yini,CHEN Xiaoyan,YANG Hongmei,et al. RP -HPLC determination of dobesilic acid in human plasma[J]. Chinese Journal of Pharmaceutical Analysis,2007,27(8):1208-1211. |
| APA | ZHANG Yini,CHEN Xiaoyan,YANG Hongmei,&ZHONG Dafang.(2007).RP -HPLC determination of dobesilic acid in human plasma.Chinese Journal of Pharmaceutical Analysis,27(8),1208-1211. |
| MLA | ZHANG Yini,et al."RP -HPLC determination of dobesilic acid in human plasma".Chinese Journal of Pharmaceutical Analysis 27.8(2007):1208-1211. |
入库方式： OAI收割
来源：上海药物研究所
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