A rapid and convenient method for preparative separation of three indissolvable polyphenols from Euphorbia pekinensis by the flexible application of solvent extraction combined with counter-current chromatography
文献类型：期刊论文
| 作者 | Gu, Jiamin2,3; Feng, Reihong3; Wu, Zhiyuan3; Guan, Shuguang1; Guo, Jiling1; Cheng, Chunru3; Xu, Pingping3; Zhang, Jingxian3; Wang, Yang3; Qu, Hua3 |
| 刊名 | JOURNAL OF SEPARATION SCIENCE
 |
| 出版日期 | 2013-07 |
| 卷号 | 36期号:14页码:2366-2372 |
| 关键词 | Counter-current chromatography Euphorbia pekinensis Indissolvable polyphenols |
| ISSN号 | 1615-9306 |
| DOI | 10.1002/jssc.201300008 |
| 文献子类 | Article |
| 英文摘要 | A rapid and convenient method was established to preparatively isolate the three ellagic acid types of compounds, which were the main polyphenols in Euphorbia pekinensis, by flexibly applying solvent extraction combined with counter-current chromatography (CCC). The total extract (extracted using 95% ethanol) of E. pekinensis was pretreated by two simple steps before CCC isolation, following the procedure: the total extract was extracted by classical solvent extraction using petroleum ether and ethyl acetate, respectively, and then the ethyl acetate extract was suspended using 95% ethanol, after being allowed to stand overnight, the sediment was obtained. Partial sediment (100 mg) was then directly separated by CCC with a two-phase solvent system composed of chloroform-95% ethanol-water-85% formic acid (50:50:50:5, v/v/v/v). About 22 mg of 3,3'-dimethoxy ellagic acid (1), 12 mg of 3,3'-di-O-methyl-4-O-(beta-D-xylopyranosyl) ellagic acid (2), and 35 mg of ellagic acid (3) with purities of 96.0, 95.2, and 95.4% were obtained respectively in one step within 4 h. After being purified by washing with methanol, the purities of the three compounds obtained were all above 98%. The purities were determined by HPLC and their chemical structures were further identified by H-1 and C-13 NMR spectroscopy. The recoveries were calculated as 84.6, 85.7, and 89.5%, respectively. The result demonstrated that the present isolation method was rapid, economical and efficient for the preparative separation of polyphenols from E. pekinensis. |
| WOS关键词 | ELLAGIC ACID ; PURIFICATION ; DERIVATIVES |
| 资助项目 | Twelfth Five-Year National Science & Technology Support Program[2012BAI29B06] ; Chinese Academy of Sciences[KSCX2-YW-R-166] ; National Science & Technology Major Project "Key New Drug Creation and Manufacturing Program," China[2011ZX09307-002-03] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000321760600019 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277553]  |
| 专题 | 成果转移转化处 |
| 通讯作者 | Guan, Shuhong |
| 作者单位 | 1.Changchun Univ Chinese Med, Changchun, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Shanghai, Peoples R China; 3.Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med,Shanghai Inst Biol Sci, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Gu, Jiamin,Feng, Reihong,Wu, Zhiyuan,et al. A rapid and convenient method for preparative separation of three indissolvable polyphenols from Euphorbia pekinensis by the flexible application of solvent extraction combined with counter-current chromatography[J]. JOURNAL OF SEPARATION SCIENCE,2013,36(14):2366-2372. |
| APA | Gu, Jiamin.,Feng, Reihong.,Wu, Zhiyuan.,Guan, Shuguang.,Guo, Jiling.,...&Guo, Dean.(2013).A rapid and convenient method for preparative separation of three indissolvable polyphenols from Euphorbia pekinensis by the flexible application of solvent extraction combined with counter-current chromatography.JOURNAL OF SEPARATION SCIENCE,36(14),2366-2372. |
| MLA | Gu, Jiamin,et al."A rapid and convenient method for preparative separation of three indissolvable polyphenols from Euphorbia pekinensis by the flexible application of solvent extraction combined with counter-current chromatography".JOURNAL OF SEPARATION SCIENCE 36.14(2013):2366-2372. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。