Simultaneous determination of atractylenolide II and atractylenolide III by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of Atractylodes Macrocephala Rhizoma extract
文献类型：期刊论文
| 作者 | Shi, Yang-yang; Guan, Shu-hong ; Tang, Ren-neng; Tao, Si-jiao; Guo, De-an
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| 刊名 | BIOMEDICAL CHROMATOGRAPHY
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| 出版日期 | 2012-11 |
| 卷号 | 26期号:11页码:1386-1392 |
| 关键词 | liquid chromatography-tandem mass spectrometry atractylenolide Atractylodes Macrocephala Rhizoma pharmacokinetics |
| ISSN号 | 0269-3879 |
| DOI | 10.1002/bmc.2709 |
| 文献子类 | Article |
| 英文摘要 | Atractylenolide II (AII) and atractylenolide III (AIII) are the major active components in Atractylodes Macrocephala Rhizoma (AMR). In this study, a sensitive, rapid and selective liquid chromatographytandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of AII and AIII in rat plasma using loliolide as internal standard (IS). After protein precipitation with ethyl acetate, the analytes were injected into an LC-MS/MS system for quantification. Chromatography was performed using a C18 column, eluting with water and acetonitrile (45:55, v/v) at 0.2?mL/min. All analytes including IS were monitored under positive ionization conditions by multiple reaction monitoring with an electrospray ionization source. The validated method was successfully applied to the pharmacokinetic study of AII and AIII in rat plasma after oral administration of AMR extract. The results provided a meaningful basis for evaluating the clinical applications of traditional Chinese medicine. Copyright (c) 2012 John Wiley & Sons, Ltd. |
| WOS关键词 | OVATA |
| 资助项目 | National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09308-005] ; National Science and Technology Major Project 'Key New Drug Creation and Manufacturing Program', China[2009ZX09304-002] ; Chinese Academy of Sciences[KSCX2-YW-R-166] ; National Natural Science Foundation of China[81001630] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000309412500014 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/277898]  |
| 专题 | 成果转移转化处 上海中药现代化研究中心 |
| 通讯作者 | Guan, Shu-hong |
| 作者单位 | Chinese Acad Sci, Shanghai Res Ctr Modernizat Tradit Chinese Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shi, Yang-yang,Guan, Shu-hong,Tang, Ren-neng,et al. Simultaneous determination of atractylenolide II and atractylenolide III by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of Atractylodes Macrocephala Rhizoma extract[J]. BIOMEDICAL CHROMATOGRAPHY,2012,26(11):1386-1392. |
| APA | Shi, Yang-yang,Guan, Shu-hong,Tang, Ren-neng,Tao, Si-jiao,&Guo, De-an.(2012).Simultaneous determination of atractylenolide II and atractylenolide III by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of Atractylodes Macrocephala Rhizoma extract.BIOMEDICAL CHROMATOGRAPHY,26(11),1386-1392. |
| MLA | Shi, Yang-yang,et al."Simultaneous determination of atractylenolide II and atractylenolide III by liquid chromatography-tandem mass spectrometry in rat plasma and its application in a pharmacokinetic study after oral administration of Atractylodes Macrocephala Rhizoma extract".BIOMEDICAL CHROMATOGRAPHY 26.11(2012):1386-1392. |
入库方式： OAI收割
来源：上海药物研究所
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