中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 上海药物研究所
  3. 中国科学院上海药物研究所
  4. 成果转移转化处
Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool

文献类型：期刊论文

作者Wu, Hao1,2; Feng, Ruihong1; Guan, Shuguang3; Yu, Wenbo3; Man, Wei3; Guo, Jiling3; Liu, Xuan1; Yang, Min1; Jiang, Baohong1; Wu, Wanying1
刊名JOURNAL OF SEPARATION SCIENCE
出版日期2012-05
卷号35期号:9页码:1160-1166
关键词High-speed counter-current chromatography (HSCCC) Organic acids Phenylpropanoid glycosides Sparganium stoloniferum
ISSN号1615-9306
DOI10.1002/jssc.201101073
文献子类Article
英文摘要A rapid high-speed counter-current chromatography (HSCCC) method was used to isolate five minor compounds from rhizome of Sparganium stoloniferum namely San Leng in Chinese, including two phenylpropanoid glycosides, sparganiaside A (1) and 1-O-feruloyl-3-p-coumaroylglycerol (2), and three aromatic acids, vanillic acid (3), p-hydroxylcinnamic acid (4), and p-hydroxybenzoic acid (5), of which, compound 1 was a new one. Five compounds were preparatively enriched at top efficiency by one-step HSCCC operation in the isolation procedure. A suitable solvent system composed of chloroformmethanolwater (4:3.5:1.8, v/v/v) was used. And the operation time was less than 4 h. The purities of compounds (15) in the enriched fractions were determined to be 75.8%, 66.3%, 90.6%, 79.9%, and 98.2%, respectively. The mean recoveries of the five compounds were 84.8%, 87.3%, 81.8%, 90.3%, and 92.7%, respectively. Compounds 14 were further purified by semi-preparative high-performance liquid chromatography (HPLC). This is the first report on the use of HSCCC as a fractionation tool for preparative isolation of minor compounds from S. stoloniferum. The method was proved to be rapid, convenient, high yield, and low cost. HSCCC was shown to be a quick and effective tool in isolation of natural products even though the compounds were not abundant.
WOS关键词SEPARATION ; CONSTITUENTS ; PURIFICATION ; L.
资助项目Chinese Academy of Sciences[2012BAI29B06] ; Chinese Academy of Sciences[KSCX2-YW-R-166]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000305133900014
出版者WILEY-V C H VERLAG GMBH
源URL[http://119.78.100.183/handle/2S10ELR8/278087]  
专题成果转移转化处
通讯作者Guan, Shuhong
作者单位1.Chinese Acad Sci, Shanghai Inst Mat Med, Natl Engn Lab TCM Standardizat Technol, Shanghai Res Ctr Modernizat Tradit Chinese Med, Shanghai 201203, Peoples R China;
2.Jilin Agr Univ, Changchun, Peoples R China;
3.Changchun Univ Chinese Med, Changchun, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Hao,Feng, Ruihong,Guan, Shuguang,et al. Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool[J]. JOURNAL OF SEPARATION SCIENCE,2012,35(9):1160-1166.
APA Wu, Hao.,Feng, Ruihong.,Guan, Shuguang.,Yu, Wenbo.,Man, Wei.,...&Guo, Dean.(2012).Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool.JOURNAL OF SEPARATION SCIENCE,35(9),1160-1166.
MLA Wu, Hao,et al."Rapid preparative isolation of a new phenylpropanoid glycoside and four minor compounds from Sparganium stoloniferum using high-speed counter-current chromatography as a fractionation tool".JOURNAL OF SEPARATION SCIENCE 35.9(2012):1160-1166.

入库方式： OAI收割

来源：上海药物研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。