Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells
文献类型：期刊论文
| 作者 | Yue, Q. -X.1,2; Song, X. -Y.1,2; Ma, C.1; Feng, L. -X.1; Guan, S. -H.1; Wu, W. -Y.1; Yang, M.1; Jiang, B. -H.1; Liu, X.1 ; Cui, Y. -J.2
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| 刊名 | PHYTOMEDICINE
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| 出版日期 | 2010-07 |
| 卷号 | 17期号:8-9页码:606-613 |
| 关键词 | Ganoderic acids Cytotoxicity Chemoproteomic 2-DE Mass spectrometry |
| ISSN号 | 0944-7113 |
| DOI | 10.1016/j.phymed.2009.12.013 |
| 文献子类 | Article |
| 英文摘要 | To elucidate the cytotoxicity mechanism of Garnoderma triterpenes, a chemoproteomic study using five purified ganoderic acids, ganoderic acid F (GAF), ganoderic acid K (GAK), ganoderic B (GAB), ganoderic acid D (GAD) and ganoderic acid AM1 (GAAM1) was conducted. GAF, GAK, GAB, GAD and GAAM1 treatment for 48 h inhibited the proliferation of HeLa human cervical carcinoma cells with IC50 values of 19.5 +/- 0.6 mu M, 15.1 +/- 0.5 mu M, 20.3 +/- 0.4 mu M, 17.3 +/- 0.3 mu M, 19.8 +/- 0.7 mu M, respectively. The protein expression profiles of HeLa cells treated with each ganoderic acid at dose of 15 mu M for 48 h were checked using two-dimensional electrophoresis (2-DE). The possible target-related proteins of ganoderic acids, i.e. proteins with same change tendency in all five ganoderic acids-treated groups compared with control, were identified using MALDI-TOF MS/MS. Twelve proteins including human interleukin-17E, eukaryotic translation initiation factor 5A (eIF5A), peroxiredoxin 2, ubiquilin 2, Cu/Zn-superoxide dismutase, 14-3-3 beta/alpha, TPM4-ALK fusion oncoprotein type 2, PP2A subunit A PR65-alpha isoform, nucleobindin-1, heterogeneous nuclear ribonucleoprotein K, reticulocalbin 1 and chain A of DJ-1 protein were identified. Ganoderic acids might exert their cytotoxicity by altering proteins involved in cell proliferation and/or cell death, carcinogenosis, oxidative stress, calcium signaling and ER stress. (C) 2009 Elsevier GmbH. All rights reserved. |
| WOS关键词 | INHIBITORY TRITERPENES ; ENDOPLASMIC-RETICULUM ; LIQUID-CHROMATOGRAPHY ; CYCLE ARREST ; CYTOTOXICITY ; CARCINOMA ; MECHANISM ; EXTRACT ; SPORES ; CANCER |
| 资助项目 | National Natural Science Foundation of China[30701077] ; Science and Technology Commission of Shanghai Municipality[2008DFA30350] |
| WOS研究方向 | Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000279656500009 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278866]  |
| 专题 | 上海中药现代化研究中心 成果转移转化处 分析化学研究室 药物安全性评价中心 |
| 通讯作者 | Cui, Y. -J. |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, Shanghai Res Ctr Modernizat Tradit Chinese Med, Beijing 100864, Peoples R China 2.Shanghai Univ Tradit Chinese Med, Coll Chinese Mat Med, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yue, Q. -X.,Song, X. -Y.,Ma, C.,et al. Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells[J]. PHYTOMEDICINE,2010,17(8-9):606-613. |
| APA | Yue, Q. -X..,Song, X. -Y..,Ma, C..,Feng, L. -X..,Guan, S. -H..,...&Guo, D. -A..(2010).Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells.PHYTOMEDICINE,17(8-9),606-613. |
| MLA | Yue, Q. -X.,et al."Effects of triterpenes from Ganoderma lucidum on protein expression profile of HeLa cells".PHYTOMEDICINE 17.8-9(2010):606-613. |
入库方式： OAI收割
来源：上海药物研究所
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