WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro
文献类型：期刊论文
| 作者 | Tong, Xian-kun3 ; Qiu, Hong1; Zhang, Xin2; Shi, Li-ping3; Wang, Gui-feng3; Ji, Fei-hong3; Ding, Hui-yong3; Tang, Wei3; Ding, Kan1; Zuo, Jian-ping3,4
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| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2010-05 |
| 卷号 | 31期号:5页码:585-592 |
| 关键词 | Dengue virus sulfated polysaccharide infection BHK cells alpha-D-glucan |
| ISSN号 | 1671-4083 |
| DOI | 10.1038/aps.2010.29 |
| 文献子类 | Article |
| 英文摘要 | Aim: To investigate the mode of action of WSS45, one sulfated derivative of an alpha-D-glucan from the Gastrodia elata Bl, on the multiplication cycle of dengue virus serotype 2 (DV2), including initial infection and intracellular replication. Methods: Virus multiplication in BHK cells were monitored by qRT-PCR, plaque reduction assay, and flow cytometry. Initial virus infection was dissected into adsorption and penetration steps by converting temperature and treating by acid glycine. Surface bound virions were detected by immunofluorescence staining for Evelope protein. Results: WSS45 effectively inhibited DV2 infection in BHK cells with an EC50 value of 0.68 +/- 0.17 mu g/mL, mainly interfered with virus adsorption, in a very early stage of the virus cycle. However, WSS45 showed no viricidal effect. Moreover, WSS45 could increase the detaching of virus from cell surface in BHK cell line. Conclusion: WSS45 exerted potent inhibitory effect on DV2 through interfering with the interaction between viruses and targeted cells. This activity was related to its molecular size. |
| WOS关键词 | HEPARAN-SULFATE ; CELLS ; GLYCOPROTEIN ; BINDING ; IDENTIFICATION ; ACTIVATION ; ENTRY |
| 资助项目 | European Union[517711] ; National Science & Technology Major Project[2009ZX09301-001] ; Science & Technology Commission of Shanghai Municipality, China[08XD14053] ; Science & Technology Commission of Shanghai Municipality, China[08410708900] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3893588 |
| WOS记录号 | WOS:000277661600009 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278903]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 药理学第三研究室 |
| 通讯作者 | Tang, Wei |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Glycochem & Glycobiol Lab, Shanghai 201203, Peoples R China; 2.Inst Ctr Dis Control & Prevent Guangdong Prov, Guangzhou 510300, Guangdong, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Mat Med, Lab Immunopharmacol, Shanghai 201203, Peoples R China; 4.Shanghai Univ Tradit Chinese Med, Lab Immunol & Virol, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Tong, Xian-kun,Qiu, Hong,Zhang, Xin,et al. WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro[J]. ACTA PHARMACOLOGICA SINICA,2010,31(5):585-592. |
| APA | Tong, Xian-kun.,Qiu, Hong.,Zhang, Xin.,Shi, Li-ping.,Wang, Gui-feng.,...&Zuo, Jian-ping.(2010).WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro.ACTA PHARMACOLOGICA SINICA,31(5),585-592. |
| MLA | Tong, Xian-kun,et al."WSS45, a sulfated alpha-D-glucan, strongly interferes with Dengue 2 virus infection in vitro".ACTA PHARMACOLOGICA SINICA 31.5(2010):585-592. |
入库方式： OAI收割
来源：上海药物研究所
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