Simultaneous analysis of isomers of escin saponins in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study after oral administration
文献类型：期刊论文
| 作者 | Wu, Xiujun2,4; Liu, Lidong2; Zhang, Mengliang2; Wu, Dan2; Wang, Yingwu2; Sun, Yantong2; Fawcett, J. Paul1; Gu, Jingkai2; Zhang, Jiwen3
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| 刊名 | JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
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| 出版日期 | 2010-04-01 |
| 卷号 | 878期号:11-12页码:861-867 |
| 关键词 | Escin la Escin lb Isoescin la Isoescin lb LC-MS/MS Pharmacokinetics |
| ISSN号 | 1570-0232 |
| DOI | 10.1016/j.jchromb.2010.02.002 |
| 文献子类 | Article |
| 英文摘要 | A rapid and sensitive bioassay based on liquid chromatography-tandem mass spectrometry (LC-MS/MS) for the simultaneous determination of four isomeric escin saponins (escin la, escin lb, isoescin Is and isoescin lb) in human plasma has been developed and validated. Sample preparation of plasma after addition of telmisartan as internal standard (I.S.) involved solid-phase extraction (SPE) on C18 cartridges. Separation was based on reversed phase chromatography using gradient elution with methanol-acetonitrile (50:50, v/v) and 10 mM ammonium acetate solution (pH 6.8). MS/MS detection in the positive ion mode used multiple reaction monitoring of the transition at m/z 1113.8 -> 807.6. Stability issues with the four saponins required the addition of formic acid to plasma samples prior to storage at -80 degrees C and analysis within 30 clays. The method was linear at concentrations up to 10 ng/mL with correlation coefficients > 0.996 for all analytes. The lower limit of quantitation (LLOQ) for all four saponins was 33 pg/mL. intra- and inter-clay precisions (as relative standard deviation) were all < 15% and accuracies (as relative error) in the range -5.3% to 6.1%. The method was successfully applied to a pharmacokinetic study of escins in healthy volunteers after oral administration of sodium aescinate tablets containing 60 mg escin saponins. (c) 2010 Elsevier B.V. All rights reserved. |
| WOS关键词 | HORSE CHESTNUT ; TRITERPENOID SAPONINS ; AESCULUS-CHINENSIS ; BIOACTIVE SAPONINS ; AESCIN ; SEEDS ; GLYCOSIDES ; EXTRACTS ; IB ; IA |
| 资助项目 | National Natural Science Foundation[30772613] ; National Key Technology RD Program[2008BAI51B03] ; Key Projects in the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period (Technical platform for preclinical pharmacokinetic studies, PR China[2009ZX09304-003] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000276652100003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278934]  |
| 专题 | 药物制剂研究中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Gu, Jingkai |
| 作者单位 | 1.Univ Otago, Sch Pharm, Dunedin, New Zealand; 2.Jilin Univ, Coll Life Sci, Res Ctr Drug Metab, Changchun 130021, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Ctr Drug Delivery Syst, Shanghai 201203, Peoples R China 4.Liaoning Univ Tradit Chinese Med, Affiliated Hosp, Shenyang 110041, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wu, Xiujun,Liu, Lidong,Zhang, Mengliang,et al. Simultaneous analysis of isomers of escin saponins in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study after oral administration[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2010,878(11-12):861-867. |
| APA | Wu, Xiujun.,Liu, Lidong.,Zhang, Mengliang.,Wu, Dan.,Wang, Yingwu.,...&Zhang, Jiwen.(2010).Simultaneous analysis of isomers of escin saponins in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study after oral administration.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,878(11-12),861-867. |
| MLA | Wu, Xiujun,et al."Simultaneous analysis of isomers of escin saponins in human plasma by liquid chromatography-tandem mass spectrometry: Application to a pharmacokinetic study after oral administration".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 878.11-12(2010):861-867. |
入库方式： OAI收割
来源：上海药物研究所
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