Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method
文献类型：期刊论文
| 作者 | Li, Shuijun1,3; Liu, Gangyi3; Jia, Jingying3; Miao, Yi3; Gu, Shuiming4; Miao, Peizhi4; Shi, Xueying2; Wang, Yiping1 ; Yu, Chen3
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| 刊名 | CLINICAL BIOCHEMISTRY
 |
| 出版日期 | 2010-02 |
| 卷号 | 43期号:3页码:307-313 |
| 关键词 | Digoxin LC-MS/MS Liquid-liquid extraction Therapeutic drug monitoring Heart failure Human serum |
| ISSN号 | 0009-9120 |
| DOI | 10.1016/j.clinbiochem.2009.09.025 |
| 文献子类 | Article |
| 英文摘要 | Objective: Here we develop a liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the determination of digoxin in serum. Design and methods: The serum samples were extracted with methyl tert-butyl ether using an isotope-labeled digoxin-d3 as internal standard. The analyte was separated on a reverse phase Capcell C18 column and detected in positive electrospray ionization multiple reaction monitoring mass spectrometry. Results: The chromatographic analysis was carried out within 3 min, but the complete analysis took longer because of the liquid-liquid extraction. The lower limit of quantification was 0.1 ng/mL for digoxin. The intra- and inter-batch precisions were less than 12%, and the bias ranged from -9.1% to 10.7%. The external quality assessment (EQA) results obtained with the LC-MS/MS method were comparable to target values. Subsequently, this method has been applied to the therapeutic monitoring of digoxin in a clinical setting. Conclusion: In this study, we have developed a rapid and reliable LC-MS/MS method for the therapeutic monitoring of digoxin in human serum. (C) 2009 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved. |
| WOS关键词 | DILUTION MASS-SPECTROMETRY ; INVESTIGATION GROUP TRIAL ; HUMAN PLASMA ; CARDIAC-GLYCOSIDES ; MORTALITY ; CHROMATOGRAPHY ; ASSAY ; HOSPITALIZATION ; REDUCTION ; MORBIDITY |
| 资助项目 | Science and Technology Commission of Shanghai Municipality, Shanghai, China[08411966700] ; Shanghai Health Bureau, Shanghai, China[0511028] |
| WOS研究方向 | Medical Laboratory Technology |
| 语种 | 英语 |
| WOS记录号 | WOS:000274104000015 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/278979]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Yu, Chen |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Drug Res, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China; 2.Shanghai Xuhui Cent Hosp, Dept Nucl Med, Shanghai 200031, Peoples R China 3.Shanghai Xuhui Cent Hosp, Cent Lab, Shanghai 200031, Peoples R China; 4.Shanghai Xuhui Cent Hosp, Dept Cardiol, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Shuijun,Liu, Gangyi,Jia, Jingying,et al. Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method[J]. CLINICAL BIOCHEMISTRY,2010,43(3):307-313. |
| APA | Li, Shuijun.,Liu, Gangyi.,Jia, Jingying.,Miao, Yi.,Gu, Shuiming.,...&Yu, Chen.(2010).Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method.CLINICAL BIOCHEMISTRY,43(3),307-313. |
| MLA | Li, Shuijun,et al."Therapeutic monitoring of serum digoxin for patients with heart failure using a rapid LC-MS/MS method".CLINICAL BIOCHEMISTRY 43.3(2010):307-313. |
入库方式： OAI收割
来源：上海药物研究所
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