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Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice

文献类型：期刊论文

作者Zhao, Hongyan1; Luo, Yingying2; Lu, Cheng3; Lin, Na7; Xiao, Cheng6; Guan, Shuhong5; Guo, De-an5; Liu, Zhenli2; Ju, Dahong1; He, Xiaojuan3
刊名PLANTA MEDICA
出版日期2010-02
卷号76期号:3页码:223-227
关键词mucosal immune response cytokines Ganoderma lucidum polysaccharide Ganoderma lucidum Polyporaceae
ISSN号0032-0943
DOI10.1055/s-0029-1186055
文献子类Article
英文摘要Ganoderma lucidum is a well-known traditional Chinese medicine and often used for improving quality of life. Ganoderma lucidum polysaccharide (GLP) is the main active component in the fungus. Recent researches have shown that oral administration of GLP could produce immunomodulation and antitumor activity. With a high molecular weight, GLP is not easy to be absorbed, and the pathway via enteric mucosal immune response might be important for the immunomodulation of GLP. To investigate the potential mechanism, Kunming mice, weighing 20 +/- 2 g, were used and their peripheral blood mononuclear cells (PBMC), intraepithelial lymphocytes (IEL) and Peyer's patches lymphocytes (PPL) were separated, and incubated with GLP at different dosages (250 mu g/mL, 125 mu g/mL, 62.5 mu g/mL, 31.25 mu g/mL). MTT, ELISA and RT-PCR were used to detect the effects of GLP on the proliferation of the lymphocytes, and expression of IL-10, IL-2 and TNF-alpha. The results showed that GLP could stimulate the proliferation of PBMC and enteric mucosal lymphocytes, and promote the production of IL-2 and IL-10. The RT-PCR results also showed a higher expression of TNF-alpha and IL-10 mRNA in the lymphocytes. The higher expression of IL-2, IL-10 and TNF-alpha from the lymphocytes was observed at GLP concentrations of 62.5 mu g/mL and 125 mu g/mL. In conclusion, enteric mucosal immune responses could be one of the important pathways for the immunomodulation activity of GLP.
WOS关键词DENDRITIC CELLS ; EXPRESSION ; INTERLEUKIN-2 ; MATURATION ; INDUCTION ; PATHWAYS ; REISHI
资助项目National Natural Science Foundation of China[30171133] ; National Natural Science Foundation of China[30825047] ; Shanghai Municipal Education Commission[E03008]
WOS研究方向Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
语种英语
WOS记录号WOS:000274392700003
出版者GEORG THIEME VERLAG KG
源URL[http://119.78.100.183/handle/2S10ELR8/278987]  
专题成果转移转化处
上海中药现代化研究中心
通讯作者Lu, Aiping
作者单位1.China Acad Chinese Med Sci, Inst Basic Theory, Beijing, Peoples R China;
2.Jiangxi Univ Tradit Chinese Med, Nanchang, Jiangxi, Peoples R China;
3.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China;
4.Shanghai TCM Univ, Shanghai Municipal Educ Commiss, E Inst, Shanghai, Peoples R China
5.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Shanghai Res Ctr TCM Modernizat, Shanghai, Peoples R China;
6.China Japan Friendship Hosp, Inst Clin Med, Beijing, Peoples R China;
7.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China;
推荐引用方式
GB/T 7714		 Zhao, Hongyan,Luo, Yingying,Lu, Cheng,et al. Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice[J]. PLANTA MEDICA,2010,76(3):223-227.
APA Zhao, Hongyan.,Luo, Yingying.,Lu, Cheng.,Lin, Na.,Xiao, Cheng.,...&Lu, Aiping.(2010).Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice.PLANTA MEDICA,76(3),223-227.
MLA Zhao, Hongyan,et al."Enteric Mucosal Immune Response might Trigger the Immunomodulation Activity of Ganoderma lucidum Polysaccharide in Mice".PLANTA MEDICA 76.3(2010):223-227.

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来源：上海药物研究所

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