2 '-Epi-2 '-O-acetylthevetin B induces apoptosis partly via Ca2+-mediated mitochondrial pathway in human hepatocellular carcinoma HepG2 cells
文献类型：期刊论文
| 作者 | Feng, Bo2; Huang, Cai-Guo2; Chen, Ruo-Hua3; Guo, Yue-Wei1 ; Jiao, Bing-Hua2
|
| 刊名 | CELL BIOLOGY INTERNATIONAL
 |
| 出版日期 | 2009-09 |
| 卷号 | 33期号:9页码:918-925 |
| 关键词 | 2 '-Epi-2 '-O-acetylthevetin B(GHSC-74) Apoptosis Ca2+ Mitochondrial membrane potential (Delta Psi m) HepG2 Bcl-2 |
| ISSN号 | 1065-6995 |
| DOI | 10.1016/j.cellbi.2009.06.013 |
| 文献子类 | Article |
| 英文摘要 | 2'-Epi-2'-O-acetylthevetin B (GHSC-74), a cardiac glycoside, can be isolated from the seeds of Cerbera manghas L. We demonstrated that GHSC-74 reduced the viability of HepG2 cells in a time-and dose-dependent manner, and efficiently induced apoptosis without significantly decreasing the viability of Chang human liver cells and Swiss albino 3T3 fibroblasts, as indicated by annexin-V/PI binding assay and Hoechst 33342 staining. In addition, stimulation of HepG2 cells with GHSC-74 induced a series of intracellular events: (1) loss of mitochondrial membrane potential; (2) sustained elevation of cytosolic [Ca2+]; and (3) downregulation of Bcl-2. BAPTA-AM, a cytosolic Ca2+ chelator, partly suppressed cell death and prevented mitochondrial membrane potential from losing in GHSC-74-treated HepG2 cells. In contrast, EGTA, an extracellular Ca2+ chelator, exhibited a weaker effect as compared to that of BAPTA-AM. Taken together, the Ca2+-mediated mitochondrial pathway was found to be involved in GHSC-74-induced HepG2 cell apoptosis. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved. |
| WOS关键词 | ENDOPLASMIC-RETICULUM ; INTRACELLULAR CALCIUM ; CYTOCHROME-C ; ION-CHANNEL ; BCL-2 ; CA2+ ; DEATH ; RELEASE ; GLYCOSIDES ; BCL-X(L) |
| 资助项目 | Ministry of Science and Technology of the People's Republic of China[2006AAD9Z447] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000269329700002 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279145]  |
| 专题 | 天然药物化学研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Huang, Cai-Guo |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China; 2.Second Mil Med Univ, Dept Biochem & Mol Biol, Shanghai 200433, Peoples R China; 3.Changhai Hosp, Shanghai 200433, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Bo,Huang, Cai-Guo,Chen, Ruo-Hua,et al. 2 '-Epi-2 '-O-acetylthevetin B induces apoptosis partly via Ca2+-mediated mitochondrial pathway in human hepatocellular carcinoma HepG2 cells[J]. CELL BIOLOGY INTERNATIONAL,2009,33(9):918-925. |
| APA | Feng, Bo,Huang, Cai-Guo,Chen, Ruo-Hua,Guo, Yue-Wei,&Jiao, Bing-Hua.(2009).2 '-Epi-2 '-O-acetylthevetin B induces apoptosis partly via Ca2+-mediated mitochondrial pathway in human hepatocellular carcinoma HepG2 cells.CELL BIOLOGY INTERNATIONAL,33(9),918-925. |
| MLA | Feng, Bo,et al."2 '-Epi-2 '-O-acetylthevetin B induces apoptosis partly via Ca2+-mediated mitochondrial pathway in human hepatocellular carcinoma HepG2 cells".CELL BIOLOGY INTERNATIONAL 33.9(2009):918-925. |
入库方式： OAI收割
来源：上海药物研究所
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