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The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor
文献类型：期刊论文
| 作者 | Yan, Jianbin; Zhang, Chi2; Gu, Min3 ; Bai, Zhiyan; Zhang, Weiguo; Qi, Tiancong; Cheng, Zhiwei; Peng, Wen; Luo, Haibin1; Nan, Fajun3
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| 刊名 | PLANT CELL
 |
| 出版日期 | 2009-08 |
| 卷号 | 21期号:8页码:2220-2236 |
| ISSN号 | 1040-4651 |
| DOI | 10.1105/tpc.109.065730 |
| 文献子类 | Article |
| 英文摘要 | Jasmonates play a number of diverse roles in plant defense and development. CORONATINE INSENSITIVE1 (COI1), an F-box protein essential for all the jasmonate responses, interacts with multiple proteins to form the SCFCOI1 E3 ubiquitin ligase complex and recruits jasmonate ZIM-domain (JAZ) proteins for degradation by the 26S proteasome. To determine which protein directly binds to jasmonoyl-isoleucine (JA-Ile)/coronatine (COR) and serves as a receptor for jasmonate, we built a high-quality structural model of COI1 and performed molecular modeling of COI1-jasmonate interactions. Our results imply that COI1 has the structural traits for binding JA-Ile or COR. The direct binding of these molecules with COI1 was further examined using a combination of molecular and biochemical approaches. First, we used the immobilized jasmonate approach to show that the COI1 protein in crude leaf extracts can bind to the jasmonate moiety of JA-Ile. Second, we employed surface plasmon resonance technology with purified COI1 and JAZ1 protein to reveal the interaction among COI1, JA-Ile, and JAZ1. Finally, we used the photoaffinity labeling technology to show the direct binding of COR with purified insect-expressed COI1. Taken together, these results demonstrate that COI1 directly binds to JA-Ile and COR and serves as a receptor for jasmonate. |
| WOS关键词 | METHYL JASMONATE ; PHYTOTOXIN CORONATINE ; UBIQUITIN-LIGASE ; AUXIN RECEPTOR ; BIOSYNTHESIS ; DEFENSE ; PATHWAY ; SIGNALS ; BINDING ; GROWTH |
| 资助项目 | National Science Foundation of China[00000000] ; The National Basic Research 973 Program of China[00000000] ; Chinese Academy of Sciences[00000000] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000270416700006 |
| 出版者 | AMER SOC PLANT BIOLOGISTS |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279178]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Xie, Daoxin |
| 作者单位 | 1.Sun Yat Sen Univ, Sch Pharmaceut Sci, Guangzhou 510006, Guangdong, Peoples R China 2.Tsinghua Univ, Dept Biol Sci & Biotechnol, Prot Sci Key Lab, Minist Educ,Sch Med, Beijing 100084, Peoples R China; 3.Chinese Acad Sci, Natl Ctr Drug Screening, State Key Lab Drug Res, Shanghai Inst Mat Med,Shanghai Inst Biol Sci, Shanghai 201203, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Yan, Jianbin,Zhang, Chi,Gu, Min,et al. The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor[J]. PLANT CELL,2009,21(8):2220-2236. |
| APA | Yan, Jianbin.,Zhang, Chi.,Gu, Min.,Bai, Zhiyan.,Zhang, Weiguo.,...&Xie, Daoxin.(2009).The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor.PLANT CELL,21(8),2220-2236. |
| MLA | Yan, Jianbin,et al."The Arabidopsis CORONATINE INSENSITIVE1 Protein Is a Jasmonate Receptor".PLANT CELL 21.8(2009):2220-2236. |
入库方式： OAI收割
来源：上海药物研究所
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