Marine-derived oligosaccharide sulfate (JG3) suppresses heparanase-driven cell adhesion events in heparanase over-expressing CHO-K1 cells
文献类型：期刊论文
| 作者 | Li, Qiu-ning; Liu, Hai-ying; Xin, Xian-liang; Pan, Qiu-ming; Wang, Lu; Zhang, Jing; Chen, Qin; Geng, Mei-yu ; Ding, Jian
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| 刊名 | ACTA PHARMACOLOGICA SINICA
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| 出版日期 | 2009-07 |
| 卷号 | 30期号:7页码:1033-1038 |
| ISSN号 | 1671-4083 |
| 关键词 | JG3 heparanase cell adhesion cell motility |
| DOI | 10.1038/aps.2009.97 |
| 文献子类 | Article |
| 英文摘要 | Aim: To elucidate the detailed mechanisms underlying the appreciable effects of JG3, a novel marine-derived oligosaccharide, on cell migration using a Chinese hamster ovary (CHO) cell line stably over-expressing heparanase. Methods: A retrovirus infection system was used to establish a CHO-K1 cell line stably transfected with heparanase. Immunocytochemistry was used to assess cell morphology. Flow cytometry was selected to analyze the activation of beta 1-integrin, and Western blotting was used to analyze the downstream effects on the cell adhesion pathway. An affinity precipitation assay was used to determine activation of the small GTPases, Rac1, and RhoA. Results: JG3 abolished heparanase-driven formation of focal adhesions and cell spreading. Although JG3 failed to block the heparanase-triggered activation of beta 1-integrin or the phosphorylation of Src, the oligosaccharide caused a significant dephosphorylation of FAK and subsequent inactivation of Erk. Furthermore, JG3 was found to arrest the activation of Rac1. Conclusion: All these findings help form an alternative view to understand the mechanisms underlying the inhibitory effects of JG3 on cell motility. |
| WOS关键词 | SUBENDOTHELIAL EXTRACELLULAR-MATRIX ; ALPHA-4-BETA-1 INTEGRIN ; DEGRADATION ; METASTASIS ; PROTEOGLYCANS ; GROWTH ; CANCER ; RHO ; ANGIOGENESIS ; FIBRONECTIN |
| 资助项目 | Natural Science Foundation of China for Distinguished Young Scholars[30725046] ; Natural Science Foundation of China for Innovation Research Group[30721005] |
| WOS研究方向 | Chemistry ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| CSCD记录号 | CSCD:3628680 |
| 出版者 | ACTA PHARMACOLOGICA SINICA |
| WOS记录号 | WOS:000268066400021 |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279190]  |
| 专题 | 药理学第一研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Geng, Mei-yu |
| 作者单位 | Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, Div Antitumor Pharmacol,State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiu-ning,Liu, Hai-ying,Xin, Xian-liang,et al. Marine-derived oligosaccharide sulfate (JG3) suppresses heparanase-driven cell adhesion events in heparanase over-expressing CHO-K1 cells[J]. ACTA PHARMACOLOGICA SINICA,2009,30(7):1033-1038. |
| APA | Li, Qiu-ning.,Liu, Hai-ying.,Xin, Xian-liang.,Pan, Qiu-ming.,Wang, Lu.,...&Ding, Jian.(2009).Marine-derived oligosaccharide sulfate (JG3) suppresses heparanase-driven cell adhesion events in heparanase over-expressing CHO-K1 cells.ACTA PHARMACOLOGICA SINICA,30(7),1033-1038. |
| MLA | Li, Qiu-ning,et al."Marine-derived oligosaccharide sulfate (JG3) suppresses heparanase-driven cell adhesion events in heparanase over-expressing CHO-K1 cells".ACTA PHARMACOLOGICA SINICA 30.7(2009):1033-1038. |
入库方式： OAI收割
来源：上海药物研究所
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