Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling
文献类型：期刊论文
| 作者 | Zhang, Xinhua2,3,4; Zhou, Zheng2; Wang, Dakui1,2; Li, Aiqun3; Yin, Yanqing2; Gu, Xiaosong1; Ding, Fei1; Zhen, Xuechu5; Zhou, Jiawei2 |
| 刊名 | JOURNAL OF NEUROSCIENCE
 |
| 出版日期 | 2009-06-17 |
| 卷号 | 29期号:24页码:7766-7775 |
| ISSN号 | 0270-6474 |
| DOI | 10.1523/JNEUROSCI.0389-09.2009 |
| 文献子类 | Article |
| 英文摘要 | Fibroblast growth factor-2 (FGF-2) is predominantly synthesized and secreted by astrocytes in adult brain. Our previous study showed that activation of classical dopamine receptor D-1 or D-2 elicits FGF-2 biosynthesis and secretion in astrocytes. Here, we report that astrocytic FGF-2 expression is also regulated by phosphatidylinositol (PI)-linked D-1-like receptor. SKF83959, a selective PI-linked D-1-like receptor agonist, upregulates the levels of FGF-2 protein in striatal astrocyte cultures in classical dopamine D-1 and D-2 receptor-independent manner. The conditional medium derived from SKF83959-activated astrocytes promoted the number of TH+ neurons in vitro. Treatment of astrocytes with SKF83959 increased intracellular calcium in two phases. Inhibition of intracellular calcium oscillation by inositol 1,4,5-triphosphate (IP3) inhibitors blocked the SKF83959-induced increase in FGF-2 expression. Moreover, intraperitoneal administration of SKF83959 reversed l-methyl-4-phenyl-l,2,3,6-tetrahydropypridine (MPTP)-induced reduction in FGF-2 expression in both the striatum and ventral midbrain and resulted in marked protection of dopaminergic neurons from MPTP-induced neurotoxicity. These results indicate that IP3/Ca2+/calmodulin-dependent protein kinase is an uncharted intracellular signaling pathway that is crucial for the regulation of FGF-2 synthesis in astrocytes. PI-linked D-1-like receptor plays an important role in the regulation of astrocytic FGF-2 expression and neuroprotection which may provide a potential target for the drug discovery in Parkinson's disease. |
| WOS关键词 | D-1 DOPAMINE AGONISTS ; FIBROBLAST-GROWTH-FACTOR ; PARKINSONS-DISEASE ; VARYING EFFICACIES ; RAT-BRAIN ; SUBSTANTIA-NIGRA ; SKF 83959 ; NEURONS ; SKF83959 ; CALCIUM |
| 资助项目 | Chinese Ministry of Science and Technology[2006AA02Z184] ; Chinese Ministry of Science and Technology[2006AA02A114] ; Chinese Ministry of Science and Technology[2007AA02Z163] ; Shanghai Metropolitan Fund for Research and Development[07DJ14005] ; Natural Science Foundation of China[30525041] ; Natural Science Foundation of China[30623003] ; Natural Science Foundation of China[30721004] ; State Key Program for Basic Research of China[2006CB500704] ; State Key Program for Basic Research of China[2009CB522201] |
| WOS研究方向 | Neurosciences & Neurology |
| 语种 | 英语 |
| WOS记录号 | WOS:000267131000016 |
| 出版者 | SOC NEUROSCIENCE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279207]  |
| 专题 | 药理学第二研究室 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Zhou, Jiawei |
| 作者单位 | 1.Nantong Univ, Sch Med, Jiangsu Key Lab Neuroregenerat, Nantong 226001, Jiangsu, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Neurosci, State Key Lab Neurosci, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Inst Biochem & Cell Biol, Mol Cell Biol Lab, Shanghai 200031, Peoples R China; 4.Nantong Univ, Sch Med, Dept Anat, Nantong 226001, Jiangsu, Peoples R China; 5.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xinhua,Zhou, Zheng,Wang, Dakui,et al. Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling[J]. JOURNAL OF NEUROSCIENCE,2009,29(24):7766-7775. |
| APA | Zhang, Xinhua.,Zhou, Zheng.,Wang, Dakui.,Li, Aiqun.,Yin, Yanqing.,...&Zhou, Jiawei.(2009).Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling.JOURNAL OF NEUROSCIENCE,29(24),7766-7775. |
| MLA | Zhang, Xinhua,et al."Activation of Phosphatidylinositol-Linked D-1-Like Receptor Modulates FGF-2 Expression in Astrocytes via IP3-Dependent Ca2+ Signaling".JOURNAL OF NEUROSCIENCE 29.24(2009):7766-7775. |
入库方式： OAI收割
来源：上海药物研究所
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