NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development
文献类型：期刊论文
| 作者 | Hu, Zhonghua2; Wang, Chaochen2; Xiao, Ying3; Sheng, Nengyin1; Chen, Yibin2; Xu, Ye2; Zhang, Liang2; Mo, Wei2; Jing, Naihe1; Hu, Gengxi2 |
| 刊名 | JOURNAL OF CELL SCIENCE
 |
| 出版日期 | 2009-04-15 |
| 卷号 | 122期号:8页码:1145-1154 |
| 关键词 | NDST1 BMP signaling Lung development |
| ISSN号 | 0021-9533 |
| DOI | 10.1242/jcs.034736 |
| 文献子类 | Article |
| 英文摘要 | Heparan sulfate proteoglycans (HSPGs) are required for various signaling pathways, one of which is the bone morphogenetic protein (BMP) signaling pathway. N-deacetylase/N-sulfotransferase-1 (NDST1) participates in synthesizing heparan sulfate (HS) chains of HSPGs, and is involved in bone and lung development. Here, we report that in spite of the redundant expression of Ndst2, Ndst3 and Ndst4 genes, Ndst1(-/-) mice display defective differentiation of lung cells and increased cell proliferation. Loss of Ndst1 in the lung enhances downstream BMP signaling in vivo. Noggin, which is an antagonist of BMP, can rescue the Ndst1(-/-) lung morphogenetic defects in explant cultures. Further studies in vitro indicated that loss of Ndst1 significantly impairs BMP internalization by decreasing BMP binding to endogenous HS. Exogenous heparin can rescue both the BMP signaling and BMP internalization abnormalities in Ndst1(-/-) lung. Thus, we propose that HS regulates BMP signaling by controlling the balance between BMP binding to HS, and that BMP receptors and NDST1-dependent modification are essential for this process. The results suggest that NDST1-dependent HS is essential for proper functioning of BMP in embryonic lung development. |
| WOS关键词 | N-DEACETYLASE/N-SULFOTRANSFERASE ; MOUSE EMBRYONIC LUNG ; BONE MORPHOGENETIC PROTEIN-4 ; BRANCHING-MORPHOGENESIS ; MOLECULAR-CLONING ; SPEMANN ORGANIZER ; GENE-EXPRESSION ; LACRIMAL-GLAND ; MAST-CELLS ; PROTEOGLYCANS |
| 资助项目 | Minster for Science and Technology[G1998051007] ; Chinese High-Tech RD Program[(863)-2001AA231011] ; Chinese High-Tech RD Program[0022Z2002] |
| WOS研究方向 | Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000264777700011 |
| 出版者 | COMPANY BIOLOGISTS LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279264]  |
| 专题 | 新药研究国家重点实验室 中科院受体结构与功能重点实验室 |
| 通讯作者 | Hu, Gengxi |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Key Lab Stem Cell Biol,Lab Mol Cell Biol, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Biol Sci, State Key Lab Mol Biol, Shanghai 200031, Peoples R China; 3.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Hu, Zhonghua,Wang, Chaochen,Xiao, Ying,et al. NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development[J]. JOURNAL OF CELL SCIENCE,2009,122(8):1145-1154. |
| APA | Hu, Zhonghua.,Wang, Chaochen.,Xiao, Ying.,Sheng, Nengyin.,Chen, Yibin.,...&Hu, Gengxi.(2009).NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development.JOURNAL OF CELL SCIENCE,122(8),1145-1154. |
| MLA | Hu, Zhonghua,et al."NDST1-dependent heparan sulfate regulates BMP signaling and internalization in lung development".JOURNAL OF CELL SCIENCE 122.8(2009):1145-1154. |
入库方式： OAI收割
来源：上海药物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。