Antioxidative effect of the herbal remedy Qin Huo Yi Hao and its active component tetramethylpyrazine on high glucose-treated endothelial cells
文献类型：期刊论文
| 作者 | Kang, Yingxiu3; Hu, Minghua1,2; Zhu, Yanhui1,2; Gao, Xin3; Wang, Ming-Wei1,2
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| 刊名 | LIFE SCIENCES
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| 出版日期 | 2009-03-27 |
| 卷号 | 84期号:13-14页码:428-436 |
| 关键词 | Traditional Chinese medicine Oxidative stress Endothelial cells Tetramethylpyrazine (TMP) Nitric oxide (NO) |
| ISSN号 | 0024-3205 |
| DOI | 10.1016/j.lfs.2009.01.003 |
| 文献子类 | Article |
| 英文摘要 | Aims: This study was designed to gain insights into the antioxidant mechanism of a Chinese herbal remedy, Qing Huo Yi Hao (QHYH), and its active components against oxidative stress induced by high glucose in endothelial cells. Main methods: Effects of QHYH on reactive oxygen species (ROS) production and nitric oxide (NO) generation were measured with the fluorescent markers H2DCF-DA and DAF-FM DA, respectively. Phosphorylation of Akt (protein kinase B)/eNOS (endothelial nitric oxide synthase) and uncoupling protein 2 (UCP2) expression were studied by Western blot techniques. Influences of QHYH and one of the active components (tetramethylpyrazine, TMP) on UCP2 expression were subsequently evaluated by quantitative real-time reverse transcription-polymerase chain reaction. Using RNA interference techniques, the involvement of UCP2 in high glucose-induced ROS production in mouse brain microvascular (bEnd.3) cells and its correlation with the antioxidant effect of QHYH were further assessed. Key findings: Our results showed that QHYH could protect endothelial cells from high glucose-induced damages, such as ROS production, down-regulation of Akt/eNOS phosphorylation and reduction of NO generation. The protective properties of QHYH were partially attributed to UCP2 mRNA/protein expression, because silence of UCP2 gene by siRNAs (small interfering RNAs) abolished such effects. A total of 28 extracts and 11 active components isolated from QHYH were functionally analyzed. Of which, TMP displayed comparable antioxidant and endothelial protective effects as QHYH. Significance: All of the data, taken together, point to some therapeutic potential of QHYH and TMP for vascular complications of diabetes. (C) 2009 Elsevier Inc. All rights reserved. |
| WOS关键词 | GLYCATION END-PRODUCTS ; UNCOUPLING PROTEIN-2 OVEREXPRESSION ; OXYGEN SPECIES PRODUCTION ; NITRIC-OXIDE PRODUCTION ; OXIDATIVE STRESS ; MEDICINE PREPARATION ; GENE-EXPRESSION ; MITOCHONDRIAL ; DYSFUNCTION ; PATHWAYS |
| 资助项目 | Ministry of Education of China[2006-024-6076] ; Ministry of Science and Technology of China[2004CB518902] ; Shanghai Municipality government[07JC14011] ; Shanghai Municipality Government[07DZ22920] |
| WOS研究方向 | Research & Experimental Medicine ; Pharmacology & Pharmacy |
| 语种 | 英语 |
| WOS记录号 | WOS:000264593500007 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279287]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Gao, Xin |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 200031, Peoples R China 2.Chinese Acad Sci, Natl Ctr Drug Screening, Shanghai, Peoples R China; 3.Fudan Univ, Zhongshan Hosp, Shanghai 200433, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Kang, Yingxiu,Hu, Minghua,Zhu, Yanhui,et al. Antioxidative effect of the herbal remedy Qin Huo Yi Hao and its active component tetramethylpyrazine on high glucose-treated endothelial cells[J]. LIFE SCIENCES,2009,84(13-14):428-436. |
| APA | Kang, Yingxiu,Hu, Minghua,Zhu, Yanhui,Gao, Xin,&Wang, Ming-Wei.(2009).Antioxidative effect of the herbal remedy Qin Huo Yi Hao and its active component tetramethylpyrazine on high glucose-treated endothelial cells.LIFE SCIENCES,84(13-14),428-436. |
| MLA | Kang, Yingxiu,et al."Antioxidative effect of the herbal remedy Qin Huo Yi Hao and its active component tetramethylpyrazine on high glucose-treated endothelial cells".LIFE SCIENCES 84.13-14(2009):428-436. |
入库方式： OAI收割
来源：上海药物研究所
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