Glucagon receptor mediates calcium signaling by coupling to G alpha(q/11) and G alpha(i/o) in HEK293 cells
文献类型：期刊论文
| 作者 | Xu, Yazhen; Xie, Xin
|
| 刊名 | JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION
 |
| 出版日期 | 2009 |
| 卷号 | 29期号:6页码:318-325 |
| 关键词 | Glucagon receptor calcium signaling [Ca2+](i) G alpha(q/11) protein G alpha(i/o) protein |
| ISSN号 | 1079-9893 |
| DOI | 10.3109/10799890903295150 |
| 文献子类 | Article |
| 英文摘要 | Glucagon induces intracellular Ca2+ ([Ca2+](i)) elevation by stimulating glucagon receptor (GCGR). Such [Ca2+](i) signaling plays important physiological roles, including glycogenolysis and glycolysis in liver cells and the survival of beta-cells. Previous studies indicated that phospholipase C (PLC) might be involved in glucagon-mediated [Ca2+](i) response. Other studies also debated whether cAMP accumulation mediated by GCGR/G alpha(s) coupling contributes to [Ca2+](i) elevation. But the exact mechanisms remain uncertain. In the present study, we found that glucagon induces [Ca2+](i) elevation in HEK293 cells expressing GCGR. Removing extra-cellular Ca2+ did not affect glucagon-stimulated [Ca2+](i) response. But depleting the intracellular Ca2+ store by thapsigargin completely inhibited glucagon-induced [Ca2+](i) response. Experiments with forskolin and adenylyl cyclase inhibtor revealed that cAMP is not the cause of [Ca2+](i) response. Further studies with G alpha(q/11) RNAi and pertussis toxin (PTX) indicated that both G alpha(q/11) and G alpha(i/o) are involved. Combination of G alpha(q/11) RNAi and G alpha(i/o) inhibition almost completely abolished glucagon-induced [Ca2+](i) signaling. |
| WOS关键词 | ISOLATED RAT HEPATOCYTES ; G-BETA-GAMMA ; PHOSPHOLIPASE-C ; INOSITOL 1,4,5-TRISPHOSPHATE ; PROTEIN-PHOSPHORYLATION ; GLUCOSE-METABOLISM ; CA2+ ; ACTIVATION ; CHANNELS ; RELEASE |
| 资助项目 | National Natural Sciences Foundation of China[90713047] ; Ministry of Science and Technology of China[2007AA02Z308] ; Ministry of Science and Technology of China[2009CB940900] ; Shanghai Commission of Science and Technology[08JC1407701] ; Shanghai Commission of Science and Technology[09DZ2291200] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000273048400004 |
| 出版者 | INFORMA HEALTHCARE |
| 源URL | [http://119.78.100.183/handle/2S10ELR8/279397]  |
| 专题 | 国家新药筛选中心 中科院受体结构与功能重点实验室 新药研究国家重点实验室 |
| 通讯作者 | Xie, Xin |
| 作者单位 | Chinese Acad Sci, State Key Lab Drug Res, Natl Ctr Drug Screening, Shanghai Inst Mat Med, Shanghai 201203, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xu, Yazhen,Xie, Xin. Glucagon receptor mediates calcium signaling by coupling to G alpha(q/11) and G alpha(i/o) in HEK293 cells[J]. JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION,2009,29(6):318-325. |
| APA | Xu, Yazhen,&Xie, Xin.(2009).Glucagon receptor mediates calcium signaling by coupling to G alpha(q/11) and G alpha(i/o) in HEK293 cells.JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION,29(6),318-325. |
| MLA | Xu, Yazhen,et al."Glucagon receptor mediates calcium signaling by coupling to G alpha(q/11) and G alpha(i/o) in HEK293 cells".JOURNAL OF RECEPTORS AND SIGNAL TRANSDUCTION 29.6(2009):318-325. |
入库方式： OAI收割
来源：上海药物研究所
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